Intracellular nickel ions (Ni2+)have been shown to cause singlestrand breaks in DMA, that were rapidly repaired, and DNAprotein cross-links, that persisted for at least 24 h following removal of extracellular ionic nickel. In this study, we have used the techniques of alkaline elution, chromatin fractionation, and sodium dodecyl sulfate:polyacrylamide gel electrophoresis to examine the DNA-prot...

The Regional Monitoring Program for Water Quality in the San Francisco Bay (RMP) has conducted annual monitoring of the San Francisco Estuary (estuary) since 1993. The RMP primarily monitors water, sediment, and bivalves, although short-term pilot and special studies on select topics are also conducted. The purpose of this article is to synthesize over 10 years of RMP nickel data and to illustr...

in the recent years, agricultural soils have received more chemicals in various forms for higher yields. this may result in the accumulation of metals in soils which subsequently may be transferred into the agricultural crops. in the present investigation, nickel content of 58 composite soil samples and also agricultural crops, is studied. ni in samples of soil and agricultural products were ex...

zinc-nickel electrodeposits have been widely adopted for surface treatment of automobile steel sheet for high corrosion resistance. in this work the effect of pulse parameters on the zn-ni alloy electrodeposits was investigated. the hardness, thickness, corrosion resistance and composition of deposits thus produced were investigated. the surface topography of the deposits was also observed in s...

The objective of this study was to assess the effects of nickel chloride on human and rainbow trout erythrocytes in vitro. The cells were incubated with 0, 0.5 and 1 mM nickel chloride for 1 h at pH 7.40 and 25 degrees C, then K(+) efflux, SO (4) (2-) uptake and GSH and GSSG concentrations were measured. In both kind of cells, "high concentration" nickel treatment increased KCl efflux with resp...

Stem cell research can lead to the development of treatments for a wide range of ailments including diabetes, heart disease, aging, neurodegenerative diseases, spinal cord injury, and cancer. OCT4 is a master regulator of self-renewal of undifferentiated embryonic stem cells. OCT4 also plays a crucial role in reprogramming of somatic cells into induced pluripotent stem (iPS) cells. Given known ...

Synthesis of the hydrogen uptake (Hup) system in Rhizobium leguminosarum bv. viciae requires the function of an 18-gene cluster (hupSLCDEFGHIJK-hypABFCDEX). Among them, the hupE gene encodes a protein showing six transmembrane domains for which a potential role as a nickel permease has been proposed. In this paper, we further characterize the nickel transport capacity of HupE and that of the tr...

Liprin-α4 was strongly induced following nickel (II) chloride exposure in a variety of cell types including BEAS-2B, A549, BEP2D and BL41 cells. Liprin-α4, a member of the Liprin alpha family, has seven isoforms but only three of these variants were detected in BEAS-2B cells (004, 201 and 202). The level of Liprin-α4 variants 201 and 004 were highly increased in BEAS-2B cells in response to nic...

HoxN, an integral membrane protein with seven transmembrane helices and a molecular mass of 33.1 kDa, is involved in high-affinity nickel transport in Alcaligenes eutrophus H16. From genetic analyses, it has been concluded that HoxN is a single-component ion carrier. To investigate this assumption, hoxN was introduced into Escherichia coli. The recombinant strain showed significantly enhanced n...