A simple EIA technique for the quantitation of red-cell-bound immunoglobulin (IgG) was devised and applied to normal and pathologic states, including autoimmune hemolytic anemia (AIHA) and other disorders. The mean red-cell-bound IgG value for normal subjects was 73.2 ± 25.8 ng/l0'o red cells (mean ± ISD). Fifteen patients with AIHA had an increased level of red-eell-bound IgG ranging from 109 ...

Chronic lymphocytic leukemia (CLL) is frequently associated with autoimmune diseases directed against constituents of the blood, including hemolytic anemia (AIHA). We hypothesized that CLL cells predispose to hematologic autoimmunity by acting as aberrant antigen-presenting cells (APCs). Initially, it was confirmed that all studied patients with AIHA secondary to CLL harbored activated helper T...

We examined the activity of peripheral blood monocytes in patients with autoimmune hemolytic anemia (AIHA) using an in vitro assay of monocyte-macrophage interaction with erythrocytes and an antibody-dependent cell-mediated cytotoxicity (ADCC) assay. The monocytes of AIHA patients in the hemolyzing period phagocytized autologous sensitized red cells and anti-D coated red cells more avidly than ...

Patients with autoimmune haemolytic anaemia (AIHA) frequently have anaemia of sufficient severity as to require a blood transfusion. However, it is impossible to find compatible blood when, as is frequently the case, the autoantibody in the patient's serum reacts with all normal red blood cells. Further, the autoantibody may mask the presence of a red cell alloantibody capable of causing a haem...

The association of systemic lupus erythematosus (SLE) with autoimmune hemolytic anemia (AIHA) is a known phenomenon. In many cases of AIHA, no autoantibody specificity is present. The patient’s serum reacts with all of the red blood cell (RBC) samples tested and the autoantibody appears to have broad specificity in the Rh blood group system. Occasionally, RBC autoantibodies demonstrate apparent...

Autoimmune hemolytic anemia (AIHA) is an unusual complication of malignancy. We diagnosed primary splenic anaplastic large cell lymphoma (ALCL) in a patient. A seven-year-old boy presented with Coombs test-positive hemolytic anemia. After a course of prednisolone therapy, a complete response for anemia was achieved. Twenty months later, in addition to severe hemolytic anemia, the patient was di...

Chronic hepatitis C virus (HCV) infection has been associated with various extrahepatic manifestations, including autoimmune cytopenias. Primary autoimmune haemolytic anaemia (AIHA) has been reported as an unusual extrahepatic manifestation. Combination therapy with pegylated interferon (PEG-IFN) and ribavirin (RBV) is the current treatment of choice for chronic HCV infection. However, this the...

The association of systemic lupus erythematosus (SLE) with autoimmune hemolytic anemia (AIHA) is a known phenomenon. In many cases of AIHA, no autoantibody specificity is present. The patient’s serum reacts with all of the red blood cell (RBC) samples tested and the autoantibody appears to have broad specificity in the Rh blood group system. Occasionally, RBC autoantibodies demonstrate apparent...

Peginterferon (Peg-IFN) alfa in combination with ribavirin represents the gold standard treatment for chronic hepatitis C, but is associated with various side effects, especially hematological abnormalities. We report here a case of severe autoimmune hemolytic anemia (AIHA) complicated by symptomatic myocardial ischemia in a patient with chronic hepatitis C during combination therapy. The worse...