OBJECTIVES As T-cell immunoglobulin and mucin domain 3 (TIM-3) is an immune regulatory molecule; its blocking or stimulating could alter the pattern of immune response towards a desired condition. Based on the unique features of nanobodies, we aimed to construct an anti-TIM-3 nanobody as an appropriate tool for manipulating immune responses for future therapeutic purposes. MATERIALS AND METHO...

We have developed a novel optical biosensor device using recombinant methyl parathion hydrolase (MPH) enzyme immobilized on agarose by metal-chelate affinity to detect organophosphorus (OP) compounds with a nitrophenyl group. The biosensor principle is based on the optical measurement of the product of OP catalysis by MPH (p-nitrophenol). Briefly, MPH containing six sequential histidines (6 × H...

Recently, increasing evidence suggests that the antihypertensive drug nifedipine acts as a protective agent for endothelial cells, and that the activity is unrelated to its calcium channel blocking. Nifedipine is unstable under light and reportedly decomposes to a stable nitrosonifedipine (NO-NIF). NO-NIF has no antihypertensive effect, and it has been recognized as a contaminant of nifedipine....

To mimic the carboxylate-rich active site of the manganese catalases more closely we introduced carboxylate groups into dimanganese complexes in place of nitrogen ligands. The series of dimanganese(III,IV) complexes of tripodal ligands [Mn(2)(L)(2)(O)(2)](3+/+/-/3-) was extended from those of tpa (1) and H(bpg) (2) to those of H(2)(pda) (3) and H(3)(nta) (4) (tpa=tris-picolylamine, H(bpg)=bis-p...

Background Complexing agents are widely used in various industrial processes and household products throughout the world. Amongst the well-known complexing agents, not only EDTA but also DTPA and NTA are widely used. Possible legislative restrictions of the use of biologically recalcitrant complexing agents brings about studies of novel and alternative products and at the same time more sophist...

The mechanism by which poliovirus infects the cell has been characterized by a combination of biochemical and structural studies, leading to a working model for cell entry. Upon receptor binding at physiological temperature, native virus (160S) undergoes a conformational change to a 135S particle from which VP4 and the N terminus of VP1 are externalized. These components interact with the membr...

15 The electroanalytical technique Absence of Gradients and Nernstian Equilibrium 16 Stripping (AGNES) has been extended by applying stripping chronopotentiometry 17 (SCP) as the reoxidation stage in the determination of the free concentration of Zn 2+ , 18 Cd 2+ and Pb 2+ . This new approach, called AGNES-SCP, has been implemented with 19 screen-printed electrodes (SPE) and the standard Hangin...

The studies on removal of Vanadium were conducted using Granulated Activated Carbon (GAC) in combination with Chelating agents. Ethylene di-amine Tetra Acetic Acid (EDTA) and Nitrilo Tri Acetic Acid (NTA) at 25oC. The adsorption Efficiency was studied in the pH range of 2-8. The results reveals that Langmuir and Freundlich isotherms are followed during adsorption process with the chelating agen...

Alternative, non-electrochemistry-based technologies for continuous glucose monitoring are needed for eventual use in diabetes mellitus. As part of a programme investigating fluorescent glucose sensors, we have developed fibre-optic biosensors using glucose/galactose binding protein (GBP) labelled with the environmentally sensitive fluorophore, Badan. GBP-Badan was attached via an oligohistidin...