Objective(s) Nonviral vector can be an attractive alternative to gene delivery in experimental study. In spite of some advantages in comparison with the viral vectors, there are still some limitations for efficiency of gene delivery in nonviral vectors. To determine the effective expression, the recombinant Escherichia coli lacZ genes were cloned into the different variants of pcDNA3.1 and the...

It is important to obtain structure-activity relationship (SAR) data across cationic lipids for the self-assembly and non-viral intracellular delivery of siRNA. The aims of this work are to carry out a SAR study on the efficiency of asymmetrical N,N-diacyl spermines in siRNA delivery and EGFP reporter gene silencing, with comparisons to selected mixtures composed of symmetrical N,N-diacyl sperm...

Background The primary goal of any recombinant protein production is to achieve successful gene transfer and expression in a target cell. There are two general categories of delivery vehicles/vectors employed in protein expression protocols. The first category includes the non-viral vectors, ranging from direct injection of DNA to complexing DNA with cationc lipds, polylysine, etc. The second c...

Gene, short hairpin RNA (shRNA) and small interfering RNA (siRNA) delivery can be particularly used for the treatment of diseases by the entry of genetic materials mammalian cells either to express new proteins or to suppress the expression of proteins, respectively. Polyamidoamine (PAMAM) StarburstTM dendrimers are used as non-viral vectors (carriers) for gene, shRNA and siRNA delivery. Recent...