The use of paraffin slides and tissue microarrays (TMA) is indispensable for translational research. However, storage of paraffin slides over time has a substantial detrimental effect on the quality and reliability of immunohistochemistry stains. Particularly affected by this issue may be any collaborative efforts where paraffin slides or TMAs are shipped to central laboratories and then 'bioba...

A method is described for examination of the larynx in the histopathology laboratory. Using a slicing machine, transverse slices of the whole larynx are obtained from which representative histological samples may be prepared. This method offers the advantages of a complete gross examination of the normal and pathological structures of the larynx supplemented by histological studies using any of...

INTRODUCTION A controversy regarding the association of Epstein-Barr virus (EBV) with breast carcinomas has recently been reported in the literature. The present study was carried out in an attempt to determine whether there is a relationship between latent infection with EBV and breast carcinomas in Jordanian females. MATERIAL AND METHODS Extraction of DNA from the archive samples of breast ...

Aims-We have previously demonstrated the abnormal localisation of expression of the CD44 gene in carcinoma cells in cryostat sections of fresh frozen tumour tissues, using radioactive in situ hybridisation (RISH). In order to facilitate further analysis of the expression of this gene in a wider range of neoplastic and non-neoplastic conditions, we have developed a technique which can visualise ...

This study was performed to detect amplification of DNA sequences on chromosomes 4p15.2 and 6q23-24, obtained from formalin-fixed, paraffin-embedded, breast-cancer tissues. The prognostic relevance of the amplification was also demonstrated. DNA from formalin-fixed, paraffin-embedded tumor and corresponding normal tissues of 53 patients with breast cancer was extracted and amplified by real-tim...

An immunoperoxidase method for the demonstration of carcinoembryonic antigen (CEA) in tissues was applied to formalin-fixed paraffin embedded and glutaraldehyde-fixed resin sections of normal human small intestine. CEA could easily be demonstrated coating the surface of the small intestine, lining the crypts, and in goblet cells, indicating its presence there in considerable concentration. At t...

Identification of prognostic and predictive genomic markers requires long-term clinical follow-up of patients. Extraction of high-quality DNA from archived formalin-fixed, paraffin-embedded material is essential for such studies. Of particular importance is a robust reproducible method of whole genome amplification for small tissue samples. This is especially true for high-resolution analytical...

In this paper an experimental investigation on the thermal storage capacity for a typical composite mixture of paraffinGraphiteCu PCM composite has been studied. Paraffin wax was used as the base phase change material (PCM) and the paraffin has been mixed with Cu turnings in different ratios 10, 50, 70 and 90 weight percentages and considered as a composite. The thermal charge and discharge cap...

Anatomical analysis of the plant tissues is an important technique used in research for studying process growth, development, adaptation to environmental stress as well characterizing gene localization. In Vietnam, most anatomical material was manually conducted which time-consuming and labor-intensive. this report, we present innovative method that can deliver high-quality rice root sectioning...

BACKGROUND The aim of the present study was to evaluate, by immunohistochemical staining, metallothionein expression in normal, benign and malignant canine apocrine gland tissues and to correlate the protein expression with the histological grade of malignancy. MATERIALS AND METHODS MT immunostaining was evaluated in 25 formalin-fixed and paraffin-embedded canine apocrine glands (2 normal and...