Background: Prioritisation of research activities for infectious disease pathogens is usually undertaken through the identification important and knowledge gaps. Research prioritisation an essential element both effective responses to outbreaks adequate preparedness. There however currently no published mapping on evidence from high consequence p...

Background: Pseudomonas aeruginosa, a multidrug-resistant Gram-negative bacterium, produces pyocyanin, virulence factor associated with antibiotic tolerance. High concentrations of royal jelly have an antibacterial effect, which may potentially overcome resistance. However, in some cases, tolerance can occur due to prolonged stress low-dose a...

Background: Recent studies have observed vectors resting predominantly outdoors in settings where anti-vector tools are extensively deployed, attributed to selection pressure from use of control tools. This present study examined if the outdoor behaviour vector population is random or indicative a consistent preference one site over other. M...

We have produced a murine monoclonal antibody (MAb), ZX10, recognizing the NTPase/helicase domain of the hepatitis C virus (HCV) nonstructural 3 protein (NS3), from which we designed a single-chain variable fragment (ScFv). The ZX10 MAb recognized a discontinuous epitope of the NTPase/helicase domain, of which the linear sequence GEIPFYGKAIPL at residues 1371 to 1382 constitutes one part. cDNAs...

The hepatitis C virus (HCV) nonstructural 3 protein (NS3) is a 70-kDa multifunctional enzyme with three known catalytic activities segregated in two somewhat independent domains. The essential machinery of a serine protease is localized in the N-terminal one-third of the protein, and nucleoside triphosphatase (NTPase) and helicase activities reside in the remaining C-terminal region. NS4A is a ...

Background: An expanded range of contraceptive methods could reduce unintended pregnancies. User preferences research is important for successful development products people want to us. This paper describes four approaches soliciting user input in different ways: 1) perspectives on method characteristics, 2) reactions development, 3) trade-offs between and 4) “blue-s...

Background: The elicitation of inconsistent health-state utility values (HSUVs) is a prevalent problem. There are two approaches to address this problem: (1) intervention during the process ensure that patients estimate consistent HSUVs; (2) no and HSUVs adjusted after fact. This paper studies three models recently proposed for adjusting some may consider unrealistic...

Background: The primary objective worldwide during the coronavirus disease 2019 (COVID-19) pandemic has been controlling transmission. However, lockdown measures used to mitigate transmission have affected human behavior and altered lifestyles, with a likely impact on chronic non-communicable diseases. More than year into pandemic, substantia...

Background: The effect of solid retention time (SRT) over cheese whey substrates in a fermentation process drives changes the composition polyhydroxyalkanoates (PHAs) obtained. Volatile fatty acids produced first step an anaerobic sequencing batch reactor were used as to produce PHA using mixed microbial cultures under aerobic dynamic feeding con...

Many positive-strand RNA viruses encode genes that can function in trans, whereas other genes are required in cis for genome replication. The mechanisms underlying trans- and cis-preferences are not fully understood. Here, we evaluate this concept for hepatitis C virus (HCV), an important cause of chronic liver disease and member of the Flaviviridae family. HCV encodes five nonstructural (NS) g...