PURPOSE To isolate, culture, and characterize goblet cells from the conjunctiva of rats. METHODS Conjunctival tissue was surgically removed from Sprague-Dawley rats. Goblet cells were then isolated from the nictitating membrane and fornix using explant cultures. Cells derived from the explants were grown and propagated in RPMI medium supplemented with 10% fetal bovine serum. They were charact...

The differentiated human airway epithelium consists of different cell types forming a polarized and pseudostratified epithelium. This is dramatically altered in chronic obstructive pulmonary disease (COPD), characterized by basal and goblet cell hyperplasia, and squamous cell metaplasia. The effect of cigarette smoke on human bronchial epithelial cell (HBEC) differentiation remains to be elucid...

Introduction: Goblet cells secrete high molecular weight glycoprotein known as mucin which forms a protective mucus blanket throughout the G.I.T. The present work characterizes histochemically different types of mucin in colonic goblet cells & makes a comparative study in animal species of different food habit. Methods:After ethical clearance tissues were taken from the proximal part of colon o...

Four different types of lectin were applied to the rabbit conjunctiva and the lacrimal sac to examine the distribution of glycoconjugates. The conjunctival epithelium is comprised of goblet cells and nongoblet cells. The mucus granules of the goblet cells were stained with soybean agglutinin (SBA), and the cell body of the nongoblet cells was labeled with concanavalin A (Con A). The glycocalyx ...

BACKGROUND Goblet cell hyperplasia (GCH) is a prominent feature in animal models of atopic asthma produced by immunisation and following multiple challenges with antigens. The aim of this study was to examine the effect of a beta(2) agonist on the development of GCH induced by the immune response. METHODS Brown Norway rats were immunised and challenged with an aerosol of ovalbumin for four we...

Goblet cells are secretory epithelial cells of mucosal tissues that confer protection from environmental agents or pathogens via expression and secretion of soluble mucins. Loss of these cells is associated with several chronic inflammatory disorders of the mucosa. Although demonstrated to transfer antigens from the luminal surface to stromal cells in the intestinal mucosa, it is not known if g...

In vivo conjunctival epithelial healing in albino rabbits was investigated by light microscopy following both n-heptanol and trephined conjunctival wounding. Reepithelialization occurred faster following n-heptanol treatment (3 days) versus trephination (6-7 days). No goblet cells were present in the migrating epithelium during reepithelialization. After 1 day of wounding, goblet cells disappea...

BACKGROUND AND AIMS Gastric metaplasia is frequently seen in biopsies of the duodenal cap, particularly when inflamed or ulcerated. In its initial manifestation small patches of gastric foveolar cells appear near the tip of a villus. These cells contain periodic acid-Schiff (PAS) positive neutral mucins in contrast with the alcian blue (AB) positive acidic mucins within duodenal goblet cells. P...

aim : to induce acute colitis progresses to chronicity in c57bl/6 mice by dextran sulfate sodium.   background : murine models are essential tools to understand ibd pathogenesis. among different types of chemically induced colitis models, the dextran sulfate sodium (dss)-induced colitis model is the most common model of ibd, due to its simplicity. patients and methods : male c57bl/6 mice 6–8 we...