Abstract The se del allele is one of the nonsecretor alleles ( ) FUT2 generated by an Alu-mediated recombination event and was first found in Indian Bombay phenotype individuals who have anti-H, anti-A, anti-B antibodies their serum. As well as antibodies, anti-H clinically significant because it causes sever hemolytic transfusion reactions. Like , 302 having a missense single nucleotide polymo...

We have developed a simple, labor-saving, inexpensive and rapid SNP genotyping method that directly uses a human hair root as the template. This single-tube genotyping method was used to successfully and reliably genotype the ADH1B and ALDH2 polymorphisms using a hair root (without DNA isolation) and the polymerase chain reaction (PCR) enzyme kit KOD FX. Since the DNA extraction step was elimin...

BACKGROUND BCR-ABL kinase domain (KD) mutation is the major mechanism contributing to suboptimal response to tyrosine kinase inhibitors (TKI) in BCR-ABL-positive chronic myeloid leukemia (CML) patients. T315I mutation, as one of the most frequent KD mutations, has been shown to be strongly associated with TKI resistance and subsequent therapeutic failure. A simple and sensitive method is thus r...

Sea stars adhere firmly but temporarily to various substrata as a result of underwater efficient adhesive secretions released by their tube feet. Previous studies showed that this material is mainly made up of proteins, which play a key role in its adhesiveness and cohesiveness. Recently, we solubilized the majority of these proteins and obtained 43 de novo-generated peptide sequences by tandem...

A reverse transcriptase-polymerase chain reaction (RT-PCR) and a single-tube multiplex PCR assay was modified for typing of dengue virus in different geographical areas of Thailand during 2000-2001. A set of primers (D1 and D2) was used to generate the RTPCR product of 511 bp in size which subsequently underwent a single-tube multiplex PCR amplification using the highly specific primers for eac...

We have developed a quantitative PCR assay (LightCycler* using the pair of primers JW58 and JW59 for the detection of the 35-fold repeated B gene of oxoplasma gondii. This real-time PCR, using fluorescence resonance energy transfert (FRET) hybridization probes, allows the quantification of . gondii with several technical requirements not previously described: i) an internal amplification contro...

A DNA program may be expressed as a sequence (not necessarily linear) of ligation, extraction and combination steps. As recognized by the researchers in the field, the extraction steps are subject to significant misclassification errors. The error accumulates through the successive extraction steps and may lead to completely wrong conclusions unless measures are taken to control the error. Many...

secondary clarifiers with large areas are widely applied in wastewater treatment plants. a pilot study was conducted to examine the possibility of applying one and two-stage inclined tube settlers instead of conventional secondary clarifiers. tube diameter in the first stage of the two-stage settler was wide as the conventional ones, but in the second stage, it was narrow to improve the efficie...

It is well known that gene expression is regulated at the level of individual cells, and more evidence is now emerging that heterogeneity of cell regulation is orders of magnitude greater than previously thought. In order to detect meaningful variations in transcription levels, it is necessary to measure gene expression at single cell levels rather than in bulk cells, where individual differenc...