background:   objective: the aim of this study was to evaluate the in vitro developmental competence of mouse vitrified pre-antral follicles in comparison to isolated pre-antral follicles derived from vitrified ovaries in the presence of alpha lipoic acid (ala).   materials and methods: pre-antral follicles derived from fresh, vitrified-warmed ovarian tissues and vitrified–warmed pre-antral fol...

Purpose. To investigate the prevalence of and experience related to infertility fertility preservation during training for United States (US) medical residents fellows. Methods. Cross-sectional online-based survey study US postgraduate Results. Respondents included 732 fellows, with highest percentage in Obstetrics & Gynecology (24.2%), Pediatrics (14.1%), Internal Medicine (13.9%). In total, o...

Abstract Study question Re-establish the cell-cell communication of isolated oocyte and granulosa cells (GCs) to mimic follicle microenvironment promote success rate in-vitro maturation (IVM). Summary answer The Metal-phenolic network (MPN) nanoparticles-mediated cell membrane modification system was used achieve self-assembly GCs on re-establishing Oocyte-GCs communication. What is known alrea...

background: in vitro maturation (ivm) of immature oocytes collected from ovary has been proposed for fertility preservation. in addition, quality of oocytes post ivm is one of the factors determining its developmental competence. by using the non-invasive polscope system, both meiotic spindle (ms) and zona pellucida (zp) can be assessed in living oocytes. objective: the aim was to investigate t...

PURPOSE To update guidance for health care providers about fertility preservation for adults and children with cancer. METHODS A systematic review of the literature published from March 2006 through January 2013 was completed using MEDLINE and the Cochrane Collaboration Library. An Update Panel reviewed the evidence and updated the recommendation language. RESULTS There were 222 new publica...

BACKGROUND Current options for female fertility preservation in the face of cytotoxic treatments include embryo, oocyte and ovarian tissue cryopreservation. However these methods are limited by the patient age, status or available timeframe before treatment and they necessitate invasive procedures. Agents which can prevent or attenuate the ovotoxic effects of treatment would provide significant...

BACKGROUND During cytoplasmic oocyte maturation, Ca(2+) currents are vital for regulating a broad range of physiological processes. Recent studies have demonstrated that DMSO and EG cause large transient increases in intracellular Ca(2+) in mouse oocytes. The CP used in vitrifying protocols also increases the intracellular calcium transient. The aim of this study is to evaluate the effects of v...