Oocyte developmental competence is one of the determining factors that in?uence outcomes an IVF cycle regarding ability a female gamete to reach maturation and be fertilized. A non-invasive approach using proteomic analysis oocyte cumulus cells (CCs) can used as method predict viability could potentially function molecular predictors for program prognosis. Our study was aimed at quantifying pro...

diminished ovarian reserve (dor) significantly decreases the success rate of the assisted reproductive technique (art). in this study, we assessed the effect of melatonin on the art outcomes in women with dor. a double-blinded, randomized, clinical trial was performed on 80 women with dor as a pilot study in shiraz, between 2014 and 2015. dor was defined as the presence of 2 of the following 3 ...

background: leukemia inhibitory factor (lif) is a 45-56 kda glycoprotein that has an important role in proliferation and embryo implantation. its effect on oocyte maturation and how to exert the function remained to be elucidated. methods: immature mice superovulated with human menopausal gonadotropin and germinal vesicle (gv) oocytes were obtained from ovary 48 hours after. gv oocytes were cul...

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background & aim: considering that antioxidants are known as effective free radicals scavenger, it may be able to improve the in vitro oocyte maturation and the fetal quality. this study was designed to determine the effect of gundelia tournefortii leaves (gte) hydro-alcoholic extract as a source of antioxidant on in vitro oocyte maturation. experimental: germinal vasicle (gv) were recovere...

background & aim: oocytes in vitro maturation is an enormously promising technology for the treatment of infertility, yet its clinical application remains limited owing to poor success rates. therefore this study was designed to determine the effect of gundelia tournefortii root hydro alcoholic extract as a source of antioxidant on in vitro oocyte maturation. experimental: in this study, th...

G2-arrested oocytes contain cdc2 kinase as an inactive cyclin B-cdc2 complex. When a small amount of highly purified and active cdc2 kinase, prepared from starfish oocytes at first meiotic metaphase, is microinjected into Xenopus oocytes, it induces activation of the inactive endogenous complex and, as a consequence, drives the recipient oocytes into M phase. In contrast, the microinjected kina...

The gap junction proteins connexin37 and connexin43 are required for ovarian folliculogenesis in the mouse. To define their respective roles in oogenesis, chimeric ovaries containing either null mutant oocytes and wild-type granulosa cells or the reverse combination were grafted to the renal capsules of immunodeficient female mice. After three weeks, the oocytes were tested for meiotic competen...

Cat oocytes have the unique ability to mature in vitro after temporary storage at 4°C which can provide opportunities to rescue oocytes from the ovaries of endangered felids after sudden death or medical ovariohysterectomy. It has been demonstrated that factors such as season, culture conditions and morphological quality of oocytes influence the meiotic competence of domestic cat oocytes. In th...

The aim of this study was to evaluate the suitability of a commercial kit for bovine embryo vitrification for cryopreserving cat oocytes and to evaluate comparatively the effects of its use with slow freezing procedure on cryotolerance in terms of morphology and oocyte resumption of meiosis. Germinal vesicle stage oocytes isolated from cat ovaries were either vitrified (n = 72) using a vitrific...