Virus-like particle-based vaccines for high-risk human papillomaviruses (HPVs) appear to have great promise; however, cell culture-derived vaccines will probably be very expensive. The optimization of expression of different codon-optimized versions of the HPV-16 L1 capsid protein gene in plants has been explored by means of transient expression from a novel suite of Agrobacterium tumefaciens b...

This note describes the architecture of our implementation of OLSRv2 in Java, and some extensions thereto.

The observed solar p-mode frequencies provide a powerful diagnostic of the internal structure of the Sun and permit us to test in considerable detail the physics used in the theory of stellar structure. Amongst the most commonly used techniques for inverting such helioseismic data are two implementations of the optimally localized averages (OLA) method, namely the Subtractive Optimally Localize...

Highly optimized tolerance (HOT) is a mechanism that relates evolving structure to power laws in interconnected systems. HOT systems arise where design and evolution create complex systems sharing common features, including (1) high efficiency, performance, and robustness to designed-for uncertainties, (2) hypersensitivity to design flaws and unanticipated perturbations, (3) nongeneric, special...

Codon usage adaptation of lytic viruses to their hosts is determinant for viral fitness. In this work, we analyzed the codon usage of adenoviral proteins by principal component analysis and assessed their codon adaptation to the host. We observed a general clustering of adenoviral proteins according to their function. However, there was a significant variation in the codon preference between th...

BACKGROUND Escherichia coli phytase is an acidic histidine phytase with great specific activity. Pichia pastoris is a powerful system for the heterologous expression of active and soluble proteins which can express recombinant proteins in high cell density fermenter without loss of product yield and efficiently secrete heterologous proteins into the media. Recombinant protein expression is infl...

materials and methods: in order to achieve this, recombinant plasmid harboring antisense sequences targeting both of pta and acka was assembled, after transfecting to the cells, its effects on the cell growth and acetate accumulation in the minimal media was assessed and compared with the control, the plasmid without antisense cassette, in presence and absence of iptg in escherichia coli bl21 (...

BACKGROUND Baeyer-Villiger monooxygenases (BVMOs) represent a group of enzymes of considerable biotechnological relevance as illustrated by their growing use as biocatalyst in a variety of synthetic applications. However, due to their increased use the reproducible expression of BVMOs and other biotechnologically relevant enzymes has become a pressing matter while knowledge about the factors go...

Peter Vanhoenacker, Walter Gommeren, Walter H.M.L. Luyten, Josée E. Leysen and Guy Haegeman 1 Unit of Eukaryotic Gene Expression and Signal Transduction, Department of Molecular Biology, University of Gent and Flanders Interuniversity Institute for Biotechnology, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. 2 Department of Biochemical Pharmacology, 3 Department of Functional Genomics, 2,3 Jan...

IEEE 802.11s wireless LAN mesh network technology is the next step in the evolution of wireless architecture. A WLAN mesh network consists of WLAN devices with relay functions that communicate directly. In this type of networks, path selection is based on two protocols: HWMP and RA-OLSR. This paper presents a detailed study of the performance of the proposed path selection algorithms for IEEE 8...