A low-melting solid is used as organic solvent for liquid-liquid extraction at elevated temperatures followed by solidification and decantation of the aqueous phase.

In order to exploit fully the biotechnological opportunities afforded by nonaqueous enzymology, the issue of often drastically diminished enzymatic activity in organic solvents compared with that in water must be addressed and resolved. Recent studies have made great strides towards elucidating causes of this phenomenon of activity loss. None of these causes is insurmountable; by designing stra...

The development of faster, simpler, inexpensive and more environmentally-friendly sample-preparation techniques is an important issue in chemical analysis. Recent research trends involve miniaturization of the traditional liquid-liquid-extraction principle by greatly reducing the acceptor-to-donor ratio. The current trend is towards simplification and miniaturization of sample preparation and d...

Polychlorinated hydrocarbons are prevalent environmental contaminants whose rates of biodegradation are limited by their minimal solubilities in aqueous solutions where the biological reactions take place. In this study, ligninase (LiP) from Phanerochaete chrysosporium was modified by poly(ethylene glycol) to enhance its activity and stability for the biodegradation of pentachlorophenol (PCP) i...

There are several reports about the optimization of protease production, but only few have optimized the production of organic solvent tolerant keratinolytic proteases that show remarkable exploitation in the development of the non-polluting processes in biotechnological industries. The present study was carried with aim to optimize the production of a thermostable organic solvent tolerant kera...

Using concentrated nitric acid as the solvent, and water as the non-solvent, one-dimensional (1D) organic nanowires can be synthesized in a simple, one-pot process with high yield. The method has broad validity to a wide range of aromatic molecules for the synthesis of derivatized organic wires.

A porous poly(L-lactic acid)/β-tricalcium phosphate (PLLA/β-TCP) composite scaffold was fabricated using a novel technique comprising powder mixing, compression molding, low-temperature treatment, and particulate leaching without any organic solvent. The effect of this scaffold on osteoblast proliferation and differentiation was evaluated in vitro. The fabricated scaffold had a homogeneously in...

A method based on the ultrasound-assisted solidification of floating drop microextraction technique was developed for the spectrophotometric and spectrofluorimetric determination of curcumin in turmeric powder. In this work a small volume of an organic solvent was floated on the surface of an aqueous solution. After sonication the organic solvent is solidified and separated. The effect of extra...