Tyrosine-sensitive 3-deoxy-D-arabino-heptulosonic acid ‘7phosphate synthetase was prepared by a relatively simple procedure from an operator constitutive (tyrOc) strain of Salmonella typhimurium in highly purified, nearly homogeneous form. It had a molecular weight of approximately 100,000 and was independent of Co2f for activity. In the reaction of D-erythrose 4-phosphate with enolpyruvate pho...

sine diphosphate (ADP), adenosine monophosphate (AMP), and orthophosphate were 31.1, 2.52, 1.39, 0.36, and 1.2 umoles/ml of platelets, respectively. Extracellular ATP, ADP, and AMP remained fairly constant and represented 4, 2, and 4%o of total adenine nucleotide content. Total adenine nucleotide content remained unchanged during the period of control incubation. Glycogen depletion was 17.8 ,mo...

The mechanisms by which adenosine triphosphate, thrombin, and trypsin cause relaxation of vascular smooth muscle were investigated. Relaxation of the rat thoracic aorta with adenosine triphosphate, thrombin, and/or trypsin was associated with increased levels of cyclic guanosine monophosphate in both time- and concentration-dependent manners. Thrombin and trypsin did not alter cyclic adenosine ...

Lead acetate treatment of unfixed cells immobilizes the intracellular water-soluble, inorganic orthophosphate ions as microcrystalline lead hydroxyapatite precipitates (see reference 1) . These precipitates have been analyzed with the electron microprobe . A much higher concentration of phosphorus has been found in the nucleoli of maize root tip cells fixed in lead acetate-glutaraldehyde (organ...

Initial velocity studies and product inhibition patterns for purine nucleoside phosphorylase from rabbit liver were examined in order to determine the predominant catalytic mechanism for the synthetic (forward) and phosphorolytic (reverse) reactions of the enzyme. Initial velocity studies in the absence of products gave intersecting or converging linear double reciprocal plots of the kinetic da...

A procedure involving alkaline extraction and solution 31P nuclear magnetic resonance (NMR) spectroscopy was developed and optimized for the characterization of P in animal manures (broiler, swine, beef cattle). Inclusion of ethylenediaminetetraacetic acid (EDTA) in the alkaline extraction solution recovered between 82 and 97% of the total P from the three manures, which represented a significa...

This study investigated the feasibility of applying an anaerobic step-feeding strategy to enhance performance granular sequencing batch reactors (GSBRs) in terms operational stability cultivated mature granules and nutrient removal efficiencies. Two identical 5 L were operated with a total cycle time 8 h. GSBRs high-strength synthetic wastewater (COD = 1250 ± 43, ammonium (NH4-N) 115.2 4.6, ort...

In surgical disciplines, where bones have to be repaired, augmented or improved, bone substitutes are essential. Therefore, an interest has dramatically increased in application of synthetic bone grafts. As various interactions among cells, surrounding tissues and implanted biomaterials always occur at the interfaces, the surface properties of the implants are of the paramount importance in det...

The title compound, SrNi2Fe(PO4)3, synthesized by solid-state reaction, crystallizes in an ordered variant of the α-CrPO4 structure. In the asymmetric unit, two O atoms are in general positions, whereas all others atoms are in special positions of the space group Imma: the Sr cation and one P atom occupy the Wyckoff position 4e (mm2), Fe is on 4b (2/m), Ni and the other P atom are on 8g (2), on...

This research is motivated by the high levels of phosphate, which can be in form orthophosphate waters where most it comes from hotel waste that contains detergents are one factors water pollution. The aims to strive for an environmental mitigation engineering system reduce or even eliminate pollution using a plant-based treatment technique known as phytoremediation. Phytoremediation uses hyaci...