Antioxidant neuroprotection: Since the elaboration of the concept of oxidative stress in the 1980s, the idea that this phenomenon may be particularly involved in diseases of the brain has become widely accepted (Halliwell, 2006). Embedded in the framework of neuro-protection, the investigation of antioxidant strategies was fuelled by the repeated observation of redox dysregulation and outright ...

A procedure is described for preparing intact mitochondria from leaves of Sedum praealtum D.C., a plant showing Crassulacean acid metabolism. These mitochondria oxidized malate, pyruvate, alpha-ketoglutarate, succinate, NADH, NADPH, and isocitrate with good respiratory control and ADP/O ratios better than those observed in mitochondria from other photosynthetic tissues.Malate oxidation was very...

Oxidation of cysteine residues to disulfides drives import of many proteins into the intermembrane space of mitochondria. Recent studies in yeast unraveled the basic principles of mitochondrial protein oxidation, but the kinetics under physiological conditions is unknown. We developed assays to follow protein oxidation in living mammalian cells, which reveal that import and oxidative folding of...

Radical mediated protein oxidation occurs during aging and some human diseases (e.g. cataracts, heart disease). Tyrosine (Tyr) and tryptophan (Trp) residues are particularly susceptible to oxidation, resulting in the formation of protein-bound Tyr and Trp radicals which can readily propagate damage to other cellular components. As protein oxidation is detrimental in biological systems, it is de...

Oxidation of methionine disrupts the structure and function of a range of proteins, but little is understood about the chemistry that underlies these perturbations. Using quantum mechanical calculations, we found that oxidation increased the strength of the methionine-aromatic interaction motif, a driving force for protein folding and protein-protein interaction, by 0.5-1.4 kcal/mol. We found t...

Soybean meal (SBM) is a premier source of protein for feeding food-producing animals. However, its nutritional value can be compromised by oxidation. In this study, total 54 sources solvent extracted SBM (SSBM) and eight mechanically (MSBM), collected from different commercial producers geographic locations in the United States during years 2020 2021, were examined chemometric analysis to deter...

The widely accepted oxidative stress theory of aging postulates that aging results from accumulation of oxidative damage. Surprisingly, data from the longest-living rodent known, naked mole-rats [MRs; mass 35 g; maximum lifespan (MLSP) > 28.3 years], when compared with mice (MLSP 3.5 years) exhibit higher levels of lipid peroxidation, protein carbonylation, and DNA oxidative damage even at a yo...

Abstract Objectives The secondary products of lipid oxidation are one the main factors inducing protein oxidation. effects treatment with malondialdehyde (MDA) on immunoreactivity amandin and its digestion were studied. Materials Methods rabbit IgG binding ability was analyzed by western blotting, changes in during different degrees investigated combination an almond allergen enzyme-linked immu...

Protein oxidative or redox modifications induced by reactive oxygen species (ROS) or reactive nitrogen species (RNS) not only can impair protein function, but also can regulate and expand protein function under a variety of stressful conditions. Protein oxidative modifications can generally be classified into two categories: irreversible oxidation and reversible oxidation. While irreversible ox...

Retinol has little effect on the activity of protein kinase C. However, air oxidation of retinol produces products which are inhibitory to this enzyme. These results are consistent with a suggestion that the activity of protein kinase C is modulated by the bulk biophysical properties of its environment. The facile susceptibility of retinol to oxidation, as demonstrated by HPLC analysis, explain...