Ca2 is an essential requirement in membrane fusion, acting through binding proteins such as calmodulin (CaM). Ca2 /CaM is required for early endosome fusion in vitro, however, the molecular basis for this requirement is unknown. An additional requirement for endosome fusion is the protein Early Endosome Antigen 1 (EEA1), and its recruitment to the endosome depends on phosphatidylinositol 3-phos...

Nicotinic acid-adenine dinucleotide phosphate (NAADP) is a novel intracellular Ca2+ releasing agent recently described in sea-urchin eggs and egg homogenates. Ca2+ release by NAADP is independent of that induced by either inositol trisphosphate (InsP3) or cyclic adenosine dinucleotide phosphate (cADPR). We now report that in sea urchin egg homogenates, NAADP releases Ca2+ from a Ca2+ pool that ...

Three dimensional printing was investigated for fabricating hydroxyapatite (HA) and tricalcium phosphate ( -TCP) composite scaffolds using calcium phosphate based ceramics and calcium phosphate cement chemistry. Scaffolds were formed by printing an aqueous sodium phosphate solution on the powder bed consisting of a mixture of dicalcium phosphate anhydrous (DCPA) and calcium hydroxide powders. T...

Ca2+ is an essential requirement in membrane fusion, acting through binding proteins such as calmodulin (CaM). Ca2+/CaM is required for early endosome fusion in vitro, however, the molecular basis for this requirement is unknown. An additional requirement for endosome fusion is the protein Early Endosome Antigen 1 (EEA1), and its recruitment to the endosome depends on phosphatidylinositol 3-pho...

Insulin-like growth factor (IGF-I) stimulates thyroid cell proliferation. Using primary cultured porcine thyroid cells, we studied the intracellular pathways that mediate the action of IGF-I on thyroid cell proliferation. IGF-I stimulates inositol phosphate accumulation, a rise in cytoplasmic free calcium [( Ca2+]i), and cell proliferation. Exposure to IGF-I results in a time- and dose-dependen...

The effect of temperature on sarcoplasmic reticulum vesicles isolated from rabbit skeletal muscle was studied by measuring the Ca2+ uptake, ATP and ITP hydrolysis, Ca2+ efflux, ATP synthesis, ATP Pi exchange reaction, and phosphorylation of the Ca 2+ transport enzyme by either ATP, ITP, or inorganic phosphate. At 0”, the enzyme is phosphorylated by ATP or ITP, but both Ca2+ uptake and P, libera...

Percentage net phosphorus availability (NPHA) and set phosphorus utilization (NPHU) of the phosphorus contained in a heat-sterilized poultry litter (PL) as compared to feed grade dicalcium phosphate (DCP) for lambs was assessed by the "slope" method. The method was based on the evaluation of the function of apparent phosphorus absorption (NPHA), or retention (NPHU), on phosphorus intake. Nitrog...

To investigate the effect of dietary fluoride (F) on some blood biochemical constituents, twenty male cross-bred calves (aged 6-8 months) were divided into equal groups and fed for 20 weeks on diets of a concentrate mixture and green maize (50:50 for the first 3 months and 40:60 during the later phase). The four dietary treatments differed only with respect to the F content of the mineral mixtu...