The kinetics of the alkylation of the essential sulfhydryl group of papain with chloroacetic acid was studied. The variation of the second order rate constant with pH at 4.5” and 30.5’, r/2 = 0.07, is bell-shaped, with rate constants at a maximum near neutrality, approaching zero at low pH, and approaching a positive minimum at high pH. These profiles are described by a formulation which relate...

Mastitis, a severe infectious disease among dairy cattle, is still a challenge for livestock treatment due to the increased infections by antibiotic resistant strains. Antimicrobial peptides could be the alternate for treating such drug resistant strains. In the present study, the whey proteins extracted from buffalo milk was subjected to enzymatic hydrolysis using trypsin, chymotrypsin, pepsin...

Abstract In Peru there are no minimization proposals for the large amount of organic waste discarded daily by economic activities such as in case El Tambo food market. present work we use Ananas comosus and Carica papaya peels seeds to obtain enzymes bromelain papain, using protocol [1] with following steps: obtaining juice, enzymatic extraction ethanol NaCl, protein concentration finally activ...

After preliminary assays, with papain, bromelain and ficin, on a range of citrulline p-nitroanilides, values of Km and kcat. for the papain-catalysed hydrolysis of three derivatives, N alpha- benzyloxycarbonylcitrulline p-nitroanilide, benzyloxycarbonylphenylalanylcitrulline p-nitroanilide and benzyloxycarbonylglycylphenylalanylcitrulline p-nitroanilide, were obtained. It is concluded that benz...

It was confirmed that the thiol protease inhibitory activities in human lung cancer extracts were significantly higher than normal lung tissue extracts. Furthermore the thiol protease inhibitor was extracted from human lung cancer tissue and purified by papain-Sepharose and Sephadex G-100 column chromatographies. The purified inhibitor inhibited papain and ficin, and its molecular weight determ...

Papain, a cysteine protease allergen with inherent adjuvant activity, induces potent IL-4 expression by T cells in the popliteal lymph nodes of mice following footpad immunization. In this study, we identify a novel, non-BCR-mediated capacity for B cells to rapidly bind and internalize papain. B cells subsequently regulate the adaptive immune response by enhancing ICOS expression on CD4(+) T ce...

The reactions of papain (EC 3.4.22.2) with substrate-derived diazomethyl ketones reported by Leary, Larsen, Watanabe & Shaw [Biochemistry (1977) 16, 5857--5861] are unusual in that (i) these reagents fail to react with low-molecular-weight thiols and (ii) the rate of reaction with the papain thiol group does not decrease to near-zero values across a pKa of 4 as the pH is decreased. Existing dat...

The intrinsic tryptophan fluorescence of active papain is greatly enhanced upon the binding of the inhibitor acetyl+ phenylalanylglycinal, and of related compounds in which the acetyl group is replaced by benzyloxycarbonyl, benzyloxycarbonylglycyl, and benzyloxycarbonylglycylglycyl. This fluorescence enhancement is largely abolished when the active site cysteine residue of papain is blocked by ...

1. The physical, chemical and enzymic properties of subfragment 1 prepared from myosin of rabbit skeletal muscle by using two different concentrations of insoluble papain were compared. 2. Subfragment 1 prepared by using a myosin/papain ratio of 2000: 1 (by wt.) migrated on electrophoresis in non-dissociating conditions as a single enzymically active band. When prepared with a myosin/papain rat...

INTRODUCTION Articular cartilage needs sulfated-glycosaminoglycans (sGAGs) to withstand high pressures while mechanically loaded. Chondrocyte sGAG synthesis is regulated by exposure to compressive forces. Moderate physical exercise is known to improve cartilage sGAG content and might protect against osteoarthritis (OA). This study investigated whether rat knee joints with sGAG depleted articula...