A low-density, high-resolution diagnostic DNA microarray comprising 38 gene targets for 13 viral causes of meningitis and encephalitis was constructed. The array has been used for the detection of multiplex PCR-amplified viruses in cerebrospinal fluid (CSF) and non-CSF specimens. A total of 41 clinical specimens were positive for echoviruses (23 samples), herpes simplex virus type 2 (4 samples)...

The aim of this research note is to prove the following new transformation formula begin{equation*} (1-x)^{-2a},_{3}F_{2}left[begin{array}{ccccc} a, & a+frac{1}{2}, & d+1 & & \ & & & ; & -frac{4x}{(1-x)^{2}} \ & c+1, & d & & end{array}right] \ =,_{4}F_{3}left[begin{array}{cccccc} 2a, & 2a-c, & a-A+1, & a+A+1 & & \ & & & & ; & -x \ & c+1, & a-A, & a+A & & end{array} right], end{equation*} wher...

‎In this paper we consider the special classes of Sonnenschein matrices‎, ‎namely the Karamata matrices $K[alpha,beta]=left(a_{n,k}right)$ with the entries‎ ‎[{a_{n,k}} = sumlimits_{v = 0}^k {left( begin{array}{l}‎ ‎n\‎ ‎v‎ ‎end{array} right){{left( {1‎ - ‎alpha‎ - ‎beta } right)}^v}{alpha ^{n‎ - ‎v}}left( begin{array}{l}‎ ‎n‎ + ‎k‎ - ‎v‎ - ‎1\‎ ‎,,,,,,,,,,k...

BACKGROUND Karyotype analysis has been the standard method for prenatal cytogenetic diagnosis since the 1970s. Although highly reliable, the major limitation remains the requirement for cell culture, resulting in a delay of as much as 14 days to obtaining test results. Fluorescent in situ hybridisation (FISH) and quantitative fluorescent PCR (QF-PCR) rapidly detect common chromosomal abnormalit...

It is widely known that cells from epithelial tumors, e.g., breast cancer, detach from their primary tissue and enter blood circulation. We show that the presence of circulating tumor cells (CTCs) in samples of patients with primary and metastatic breast cancer can be detected with an array of selected tumor-marker-genes by reverse transcription real-time PCR. The focus of the presented work is...

The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...

Previous reports indicated the significance of the TGF-β signaling in the pathogenesis of systemic sclerosis. We tried to evaluate the possibility that microRNAs (miRNAs) play a part in the type I collagen upregulation seen in normal fibroblasts stimulated with exogenous TGF-β and systemic sclerosis (SSc) fibroblasts. miRNA expression profile was evaluated by miRNA PCR array and real-time PCR. ...

Oral health-related quality of life (OHRQoL) is a multidimensional construct that involves subjective evaluation of an individual's oral health. Although it is difficult to evaluate OHRQoL biologically, recently, it has been reported that circulating microRNAs (miRNAs) in several body fluids could reflect various health conditions. The aim of this pilot study was to investigate whether salivary...

antenna structures with low side lobe levels are normally the main goal during antenna design. in this paper, a new method for reduction of radiation pattern side lobe level in phased array antenna with uniform and non-uniform weighting is presented. in this method, by changing the array elements weight or adding several elements, such as slc technique, the pattern with low side lobe level can ...

The main cause of death for colorectal cancer (CRC) patients is the development of metastatic lesions at sites distant from the primary tumor. Therefore, it is important to find biomarkers that are related to the metastasis and to study the possible mechanisms. Recent data have shown that soluble attractant molecules called chemokines support the ...