Because both Babesia microti and Borrelia burgdorferi can be transmitted by the bite of a single coinfected Ixodes scapularis tick, an attempt was made to determine frequency with which whole blood samples that tested positive for B. infection polymerase chain reaction (PCR) would also test PCR infection. Over 7-year period from 2013 2019, 119 different patients on at least one sample. Among 11...

A Short History of the Polymerase Chain Reaction. In the 1950s, the discovery of PCR is the subject of claim and counterclaim that has yet to be.Methods Mol Biol. A short history of the polymerase chain reaction. Author information: 1Division of Cancer and.A Short History of the Polymerase Chain Reaction. Full Text, Download PDF 103K. And although this does risk overstating the impact of PCR ou...

background: typhoid fever is still one of the serious public health problems in many geographic areas and is endemic in most countries. aim of current study was to evaluate a shortened time –multiplex pcr for rapid detection of different sal monella enterica serovars. methods: the pcr primers for three target genes tyv, prt and inva were subjected for amplification by pcr. by using sim ple dna ...

BACKGROUND Warfarin is the most commonly prescribed oral anticoagulant medication but also is the second leading cause of emergency room visits for adverse drug reactions. Genetic testing for warfarin sensitivity may reduce hospitalization rates, but prospective genotyping is impeded in part by the turnaround time and costs of genotyping. Microfluidics-based assays can reduce reagent consumptio...

Toward the goal of reducing diagnostic false positives while retaining high sensitivity, a closed-tube nested PCR procedure has been developed for detecting low-copy-number human immunodeficiency virus (HIV) gag target DNA sequences. Master mix for amplification 2, in a hanging gel matrix at the reaction tube top, remains sequestered from the reaction space of the tube during amplification 1. A...

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S i n c e its discovery in 1985, the polymerase chain reaction (PCR) has had a profound impact on detecting genetic and infectious diseases, identifying new genes, and unraveling the mysteries of protein-ligand recognition.(1-s) Its universal utility is due to the exquisite specificity of amplification and ease of cycling made possible by the cloning and careful characterization of a thermostab...

Chlamydia trachomatis now is one of the most prevalent bacteria sexually transmissible diseases (STD), and as such, constitutes a serious public health problem. Diagnosis of Chlamydial infections is based on isolation of bacteria in tissue culture media that requires at least 48 to 72h. The aim of this study was the detection of Chlamydia trachomatis, using polymerase chain reaction (PCR), beca...

The use of reverse transcriptase coupled with the polymerase chain reaction (RT-PCR) has been proven to be a valuable tool in the synthesis and isolation of cDNA clones as well as the quantification of rare messenger RNA species. While a number of different approaches can be used to permit gene-specific cDNA synthesis and amplification, a recurring problem is one of high background signal (i.e....