The hemibiotrophic basidiomycete fungus Moniliophthora perniciosa, the causal agent of Witches' broom disease (WBD) in cacao, is able to grow on methanol as the sole carbon source. In plants, one of the main sources of methanol is the pectin present in the structure of cell walls. Pectin is composed of highly methylesterified chains of galacturonic acid. The hydrolysis between the methyl radica...

1. Kinetic studies of ethanolamine ammonia-lyase formation by Escherichia coli suggested that coenzyme B12 (5'-deoxyadenosylcobalamin), with ethanolamine, is a co-inducer. 2. Enzymic and immunological tests failed to show the formation of complementary enzyme components induced separately by ethanolamine and cobalamin respectively. 3. Although specific for ethanolamine as the substrate, enzyme ...

berangan banana were treated with hot water at 50 ºc for 0 (control), 10, and 20 min and then kept at room temperature (25±2 ºc), 75-80% rh to observe the ripening progress. firmness and activity of cell wall degradation enzymes such as polygalacturonase (pg), pectin methylesterase (pme), and pectate lyase (pl) were determined for treated and untreated banana. the microstructure of banana peel ...

BACKGROUND Adenylosuccinate lyase is an enzyme that plays a critical role in both cellular replication and metabolism via its action in the de novo purine biosynthetic pathway. Adenylosuccinate lyase is the only enzyme in this pathway to catalyze two separate reactions, enabling it to participate in the addition of a nitrogen at two different positions in adenosine monophosphate. Both reactions...

Pectin rich fruit juices need enzymatic treatment for clarification. As fruits are rich in pectin, pectinolytic treatment can efficiently reduce viscosity resulting in clarified fruit juice. Enzymatic treatment of mango pulp results in 80% recovery of total juice present in the fruit. Use of pectin degrading polygalacturonases increases both fruit juice extraction and clarification. Polygalactu...

A glucuronan lyase extracted from Sinorhizobium meliloti strain M5N1CS was purified to homogeneity by anion-exchange chromatography. The purified enzyme corresponds to a monomer with a molecular mass of 20 kDa and a pI of 4.9. A specific activity was found only for polyglucuronates leading to the production of 4,5-unsaturated oligoglucuronates. The enzyme activity was optimal at pH 6.5 and 50 d...

A large number of bacteria and fungi secrete extracellularly the enzyme complex often termed 'pectinase', which causes the rapid disintegration of plant tissue and is consideredtheprimaryinstrument of the attack on plant tissues by these microorganisms. The action of pectinase on a solution of pectin results in the following changes: (a) a rapid lowering of the viscosity of the solution; (b) a ...