Kluyvera citrophila penicillin G acylase (KcPGA) has recently attracted increased attention relative to the well studied and commonly used Escherichia coli PGA (EcPGA) because KcPGA is more resilient to harsh conditions and is easier to immobilize for the industrial hydrolysis of natural penicillins to generate the 6-aminopenicillin (6-APA) nucleus, which is the starting material for semi-synth...

The hydrolysis of several phenylacetylamino compounds was studied using a purified preparation of E. coli penicillin acylase. The L-isomers of phenylacetyl amino acids were cleaved much faster than the D-isomers. The same observations was made for some phenylacetylamino beta-lactams. When the beta-lactam ring is incorporated in a penam or cephem ring system, the D-isomers were hydrolysed somewh...

individual approaches to the diagnosis and management of penicillin allergy are practiced    by clinicians.   this cross-sectional survey of physicians was aimed at exploring their ways of dealing with diagnosis and management of penicillin g allergy.   of the 235 respondents, 63% believed patients’ self-reported history of penicillin allergy and avoided using penicillin g;     97% do so for pa...