In this study, we reported the feasibility of using papaya skin extract (Carica papaya L.) as an alternative to enzyme pepsin in harvesting Gnathostoma spinigerum third-stage larvae. From experimental digestion, we found that the different numbers of recovered larvae between papaya skin extract and pepsin were not statistically significant (p >0.05). When the derived larvae from pepsin and papa...

In the evolution of the digestive system of the mammal, the gastric mechanism appears to be the final and most recent addition to the alimentary armamentarium. It is found in all mammals and nearly all vertebrates. Its reported occurrence in lower animals is rare and scattered, perhaps even open to serious question. This statement is not in accord with much of the older literature. Pepsin has b...

It has been shown by the work presented in this paper that it is possible to dephosphorylate enzymically pepsin and pepsinogen with a variety of phosphatases. With the aid of a phosphodiesterase and the prostate phosphatase it has been established that the phosphorus in the two proteins is present as a diester and connects two sites of the peptide chain in a cyclic configuration. Removal of the...

The first paper of this series (1) described an electrophoretic demonstration of a complex between pepsin and bovine serum albumin during the course of the enzymatic hydrolysis of the latter. Several independent lines of evidence were presented that this complex is the Michaelis-Menten complex capable of being activated to give rise to reaction products. This communication reports the results o...

A study of the kinetics of the transformation of swine pepsinogen into pepsin under a variety of conditions has been made. The results show that the transformation as a whole is essentially autocatalytic in nature under all conditions. Evidence is presented to show the existence of a compound intermediate between pepsinogen and pepsin. This compound was found to be a reversibly dissociable comp...