The peripheral leukocyte migration inhibition test has been used to assess cellular immunity to soluble antigen extracts of breast cancer in patients and normal controls. In sequential tests over several weeks, 23 of 23 patients with breast cancer in remission reacted intermittently, with 67 of 139 tests (48%) being positive (greater than or equal to 20% migration inhibition). Similarly, 6 of 1...

The objective of this work is to use a novel soft computing approach predict the anti-inflammatory effect bio copper nanoparticles. Using modified technique, various doses Musa sapientum extract and nanoparticles were examined for their capabilities. Protein denaturation was evaluated, an inhibition percentage computed. outcomes demonstrated that quantity raised percentage, indicating greater e...

Japanese encephalitis virus (JEV) and the four dengue viruses (DENV1-4) are co-distributed in Southeast and South Asia. Since JEV is antigenically cross-reactive with DENV1-4, the differentiation between these viruses using antibody assays may be difficult. Herein, we describe the development of an epitope-blocking enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody specific f...

Methotrexate (MTX) and mercaptopurine (MP) are widely used antileukemic agents that inhibit de novo purine synthesis (DNPS) as a mechanism of their antileukemic effects. To elucidate pharmacodynamic differences among children with acute lymphoblastic leukemia (ALL), DNPS was measured in leukemic blasts from newly diagnosed patients before and after therapy with these agents. Patients were rando...

Methotrexate (MTX) and mercaptopurine (MP) are widely used antileukemic agents that inhibit de novo purine synthesis (DNPS) as a mechanism of their antileukemic effects. To elucidate pharmacodynamic differences among children with acute lymphoblastic leukemia (ALL), DNPS was measured in leukemic blasts from newly diagnosed patients before and after therapy with these agents. Patients were rando...

AKT, also referred to as protein kinase B (PKB or RAC), plays a critical role in controlling cell survival and apoptosis. To gain insights into the mechanisms regulating sperm survival after ejaculation, the role of AKT was investigated in stallion spermatozoa using a specific inhibitor and a phosphoflow approach. Stallion spermatozoa were washed and incubated in Biggers-Whitten-Whittingham med...

introduction: it was shown in a previous study, that mcpip, a cilostamide derivative, increased atrial contraction force without changing contraction rate. to improve this property, two new derivatives of cilostamide were synthesized and their effects on pde3 activity and isolated rat atria contraction were investigated. methods: the inhibitory effect of each compound on pde3 enzyme was determi...

The inhibition of in-vitro calcium-oxalate crystal formation by Phyllanthus niruri L. extract was investigated by different methods i.e. nucleation assay and synthetic urine assay. In nucleation assay, the aim was to evaluate the effectiveness of different concentrations of the extract on calcium oxalate crystallization in vitro while in synthetic urine method the percentage inhibition and grow...