Extraction with 0 04% (w/v) Triton X-100 removes the flagellar membrane from sea urchin sperm while leaving the motile apparatus apparently intact When reactivated in a suitable medium containing exogenous adenosine triphosphate (ATP), nearly 100% of the sperm are motile and they swim in a manner resembling that of live sperm. Under standard conditions, with 1 mM ATP at 25 degrees C, the reacti...

Considerable scope exists for the use of AI in camel breeding programmes but the technology is not fully developed. The fertility of both fresh and liquid-stored (24 h; 4°C) dromedary camel semen diluted in either Green Buffer (GB) or INRA-96 (INRA) was assessed. Different insemination doses were investigated: 150 and 300 x 10 motile sperm for fresh sperm, and 150, 300 and 600 x 10 motile sperm...

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Total motile sperm count and DNA fragmentation Index are two parameters in analysis that have recently been used to determine the outcome of management cases male infertility. Motile Sperm Count is one values considered better than 2010 World Health Organization standard terms predictive value for success spontaneous ongoing pregnancy rate. High Fragmentation were associated with lower an incre...

Addit ion of either 25% or 50% bovine follicular fluid (FF) to Krebs Ringer phosphate buffer, with .5% fructose added (KRPF), maintained higher percentages (P<.05) of motile sperm throughout 9-hr incubation at 37 C than did 100% KRPF or 100% FF. Accelerated sperm moti l i ty and head-to-head agglutination of sperm cells were consistently observed in all diluents that contained FF. The percentag...

Larval stages are among those most vulnerable to ocean acidification (OA). Projected atmospheric CO2 levels for the end of this century may lead to negative impacts on communities dominated by calcifying taxa with planktonic life stages. We exposed Mediterranean mussel (Mytilus galloprovincialis) sperm and early life stages to pHT levels of 8.0 (current pH) and 7.6 (2100 level) by manipulating ...

Canine epididymal sperm was collected from the cauda epididymis using 2 different methods (flushing and mincing) to compare the qualities (the percentage of progressively motile, viable, morphologically abnormal, immature and intact acrosomes) before and after freezing and thawing. No significant difference was noted in the quality of the cauda epididymal sperm immediately after collection and ...

Background: Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoosper...

This investigation was designed to determine the effects of administration of honey on sperm parameters and fertility potential in adult male Wistar rats. A total of forty five rats (thirty males and fifteen females) weighing between 225±5g were used for the study. The male rats were divided into 3 groups i.e., 2 experimental and 1 control groups of ten rats each, (n=10). The 15 female rats wer...

Objectives: The present study was conducted to evaluate the relationship between testicular volume measured by using Ultrasonography and seminogram to determine the testicular function in male infertility in Mysore, Karnataka, India. Materials and methods: A total of 274 infertile and 130 normozoospermic males were recruited for this study from Mysore, India. The testicular volumes of all subje...