*Corresponding author: Mailing address: Department of Microbiology, Aichi Prefectural Institute of Public Health, 7-6, Nagare, Tsujimachi, Kita-ku, Nagoya, Aichi 462-8576, Japan. Tel: +81-52-910-5674, Fax: +81-52-913-3641, E-mail: tyamashita@ hi-ho.ne.jp Herpangina is caused primarily by coxsackievirus A (CVA) 1, 2, 3, 4, 5 (CV-A5), 6 (CV-A6), 8, 10 (CV-A10), and 22 (1) in the genus Enterovirus...

In Hevea brasiliensis, the rubber particle in the laticiferous vessel is the site of rubber (cis-1-4-polyisoprene) biosynthesis. A 14 kilodalton protein, rubber elongation factor (REF), is associated with the rubber particle in a ratio of one REF to one rubber molecule (Dennis M, Henzel W, Bell J, Kohr W, Light D [1989] J Biol Chem 264: 18618-18628; Dennis M, Light D [1989] J Biol Chem 264: 186...

Nucleotide binding site leucine-rich repeats (NBS-LRR) accounting for the main disease resistance proteins play an important role in plant defense against pathogen attack. The current study aimed to identify new NBS-LRR gene members in native types of cucurbit species in Iran. Accordingly, DNAs of melon, cucumber and cantaloupe native types to Iran were identified using three primer pairs. PCR ...

UNLABELLED South American oil-palm (Elaeis oleifera) is not cultivated in tropical countries like Malaysia on large scale due to low yield of palm oil derived from its fruit mesocarp. However, its fruit mesocarp oil contains about 68.6 % oleic acid (C(18:1)) which is more than double in comparison to commercially cultivated oilpalm, E. guineensis Jacq Tenera (hybrid of Dura (♀) x Pisifera (♂))....

We have shown previously that COS-1 cells infected with a vaccinia virus recombinant (vTF7-3) which expresses the T7 RNA polymerase gene and then transfected with four pGEM-derived plasmids encoding the influenza A virus core proteins (nucleoprotein, PB1, PB2, and PA polypeptides) can express a synthetic influenza virus-like chloramphenicol [correction of chloramphenical] acetyltransferase (CAT...

Accession Number. U15731. Species. Bos taurus. Origin of Clone. Hybridizing with a 220-bp cDNA of the somatotropin receptor (Hauser et al., 1990) 10 clones were identified from a bovine genomic library (EMBL3 SP6pT7 cloning vector; Clontech Laboratories, Palo Alto, CA). A 2.9-kb XhoIIAatII fragment containing the promoter and exon I was subcloned into pGEM-7Zf (Promega, Madison, WI), and both s...

BACKGROUND Permanent antigenic variation of influenza viruses causes a major concern to develop an effective human influenza vaccine. Conserved antigens are new vaccine candidates because it is not necessary to match the prepared vaccine with circulating strains. Ion channel M2 protein is conserved among all influenza A viruses, allowing the virus to enter host cells. OBJECTIVES To prepare an...

To develop a plant expression system for the production of the human papillomavirus type 16 (HPV16) vaccine, we investigated whether the HPV16 L1 protein can be expressed in tobacco plants and whether it can be used as the cheapest form of edible vaccine. The HPV16 L1 coding sequence was amplified by PCR using specific primers from the plasmid pGEM-T-HPV16 containing the template sequence, and ...