A combination of narrow range isoelectric focusing, electrophoresis on polyacrylamide gel, and gel exclusion on Sephadex reveals the presence of at least 9, possibly 11 isoenzymes of phospholipase A in Naja naja (cobra) venom. Their isoelectric points range from pH 4.60 to 5.66 and their molecular weights from 8,500 to 20,200. The most abundant form (isoelectric point = pH 4.95) has been isolat...

The aim of this study was to evaluate the pH and antimicrobial activity of micro or nanoparticulate zinc oxide (ZnO) pastes with or without calcium hydroxide (CH). The following medications were evaluated: microparticulate ZnO + polyethylene glycol (PEG) 400; nanoparticulate ZnO + PEG 400; PEG 400; CH + microparticulate ZnO + PEG 400 and CH + nanoparticulate ZnO + PEG 400. The pH was assessed b...

Continuous recording of ruminal pH in cannulated cattle has been practiced to study rumen metabolism. However, most systems reported did not permit animal mobility during pH recording. Therefore, the objective of this study was to develop a continuous rumen pH data acquisition system that permitted animal mobility during data acquisition. A further objective was to compare the pH readings obtai...

Experimental All chemicals and oligonucleotides were purchased from Sigma-Aldrich. Oligonucleotides were purified by reverse phase column by Sigma-Aldrich and characteristic mass spectroscopy data is provided in table S1. UV spectroscopy was carried out using an Agilent Cary 100 with a temperature controlled 6 x 6 cell changer. CD spectra were collected on beamline B23 at Diamond Light Source. ...

Study objectives: Hypertonic saline solution inhalation is suspected to produce airway inflammation. Design: The aim of this study was to verify this hypothesis by measuring inflammatory markers in exhaled breath condensate (EBC) collected before and after sputum induction with hypertonic and isotonic saline solution. Patients and methods: We enrolled 10 patients with asthma, 10 patients with C...

Introduction: Development of new pulp capping agents has paved the way towards the preservation of pulp vitality, which is an important goal in restorative dentistry. This study sought to assess the calcium ion release, pH and setting of mineral trioxide aggregate (MTA) Angelus, an experimental formulation of nano-hybrid MTA containing nano-SiO2, nano-Al2O3 and nano-TiO2 and MTA Angelus plus na...

OBJECTIVE. The purpose of this study is to evaluate the ability of two commercial strip tests and laboratory titration to detect saliva buffer capacity. MATERIALS AND METHODS. Sixty-four patients were examined. Stimulated saliva was collected and buffer capacity was determined with two different chair-side strip tests in addition to immediate transportation to the laboratory to check the buffer...

A theoretical procedure of measuring the resistance offered by a proton exchange membrane to proton flow through it has been outlined. The process has been modeled using simple mass transfer laws and electrical analogies. The final result showed that a second order differential equation, incorporating the membrane resistance, described the process completely. A major assumption that has been ma...

A potentiometric analyzer, developed to directly measure potassium and sodium concentrations, was evaluated on aqueous solutions, whole blood, serum, and plasma. The analyzer consists of three electrodes-sodium, potassium, and reference-mounted in a fIowcell configuration, and a modified digital pH meter, which displays the ion concentrations directly. Volume of the cell is about 100 l. The ele...

BACKGROUND The diagnostic utility of saliva is currently being explored in various branches of dentistry, remarkably in the field of caries research. This study was aimed to determine if assessment of salivary pH and buffering capacity would serve as reliable tools in risk prediction of early childhood caries (ECC). MATERIALS AND METHODS Paraffin-stimulated salivary samples were collected fro...