نتایج جستجو برای: phage library

تعداد نتایج: 138241  

2013
Andrew Yee Fen-Lai Tan David Ginsburg

von Willebrand factor (VWF) tethers platelets to sites of vascular injury via interaction with the platelet surface receptor, GPIb. To further define the VWF sequences required for VWF-platelet interaction, a phage library displaying random VWF protein fragments was screened against formalin-fixed platelets. After 3 rounds of affinity selection, DNA sequencing of platelet-bound clones identifie...

2016
Xiaoli Cui Jiajia You Li Sun Xiaojing Yang Tian Zhang Kechong Huang Xuewei Pan Fenjiao Zhang Yang He Hongjiang Yang

The underlying mechanisms of phage-host interactions largely remained to be elucidated. In this work, Pseudomonas aeruginosa phage C11 was first characterized as a Myoviridae virus having a linear dsDNA molecule of 94109 bp with 1173 bp identical terminal direct repeats (TDR). Then the mutants resistant to phage C11 were screened in a Tn5G transposon mutant library of P. aeruginosa PAK, includi...

Journal: :Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan 2017
Yuji Ito

As an alternative to hybridoma technology, the antibody phage library system can also be used for antibody selection. This method enables the isolation of antigen-specific binders through an in vitro selection process known as biopanning. While it has several advantages, such as an avoidance of animal immunization, the phage cloning and screening steps of biopanning are time-consuming and probl...

Journal: :Journal of biochemical and biophysical methods 2006
Zhigang Guo Feng Bi Yanchun Tang Jing Zhang Dawen Yuan Zhinan Xia Jian-Ning Liu

The work was to explore the feasibility of protein affinity purification using ligand isolated from phage library. Reteplase was used as the model protein and a humanized semi-synthetic single chain fragment variable phage library as the source of ligand. After four rounds of biopanning, reteplase-specific phage clones were greatly enriched. The scFv gene from the best phage clone was inserted ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2001
M A Huie M C Cheung M O Muench B Becerril Y W Kan J D Marks

The ability to isolate fetal nucleated red blood cells (NRBCs) from the maternal circulation makes possible prenatal genetic analysis without the need for diagnostic procedures that are invasive for the fetus. Such isolation requires antibodies specific to fetal NRBCs. To generate a panel of antibodies to antigens present on fetal NRBCs, a new type of nonimmune phage antibody library was genera...

Journal: :The Journal of biological chemistry 2011
Tarsis F Gesteira Vivien J Coulson-Thomas Alessandro Taunay-Rodrigues Vitor Oliveira Bryan E Thacker Maria A Juliano Renata Pasqualini Wadih Arap Ivarne L S Tersariol Helena B Nader Jeffrey D Esko Maria A S Pinhal

N-Deacetylase-N-sulfotransferase 1 (Ndst1) catalyzes the initial modification of heparan sulfate and heparin during their biosynthesis by removal of acetyl groups from subsets of N-acetylglucosamine units and subsequent sulfation of the resulting free amino groups. In this study, we used a phage display library to select peptides that interact with Ndst1, with the aim of finding inhibitors of t...

Journal: :Journal of bioinformatics and computational biology 2014
Beibei Ru Peter A. C. 't Hoen Fulei Nie Hao Lin Feng-Biao Guo Jian Huang

Phage display can rapidly discover peptides binding to any given target; thus, it has been widely used in basic and applied research. Each round of panning consists of two basic processes: Selection and amplification. However, recent studies have showed that the amplification step would decrease the diversity of phage display libraries due to different propagation capacity of phage clones. This...

Journal: :Advanced pharmaceutical bulletin 2016
Leila Rahbarnia Safar Farajnia Hossein Babaei Jafar Majidi Bahman Akbari Shiva Ahdi Khosroshahi

Purpose: EGFRvIII as the most common mutant variant of the epidermal growth factor receptor is resulting from deletion of exons 2-7 in the coding sequence and junction of exons 1 and 8 through a novel glycine residue. EGFRvIII is highly expressed in glioblastoma, carcinoma of the breast, ovary, and lung but not in normal cells. The aim of the present study was identification of a novel single c...

Journal: :Protein engineering, design & selection : PEDS 2011
Isidro Hötzel Vicki Chiang Jingyu Diao Homer Pantua Henry R Maun Sharookh B Kapadia

The application of phage display technology to mammalian proteins with multiple transmembrane regions has had limited success due to the difficulty in generating these proteins in sufficient amounts and purity. We report here a method that can be easily and generally applied to sorting of phage display libraries with multispan protein targets solubilized in detergent. A key feature of this appr...

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