Human gingival fibroblasts (HGFs) degrade collagen fibrils in physiological processes by phagocytosis. Since Treponema denticola outer membrane (OM) extract perturbs actin filaments, important structures in phagocytosis, we determined whether the OM affects collagen phagocytosis in vitro by HGFs. Phagocytosis was measured by flow cytometric assessment of internalized collagen-coated fluorescent...

A timely and efficient removal of apoptotic cells their fragments is essential to maintain tissue homeostasis in normal and pathological conditions. Since the substrates is executed by endowed with phagocytic activity, issue of its regulation particular interest. In this work, we studied the effect two biologically active substances, insulin ganglioside GM1, on activity rat primary astrocytes. ...

Biocompatible dual-modality imaging nanoprobes were synthesized by the electrostatic assembly of poly(γ-glutamic acid)[Gd-DTPA] and chitosan[IRDye800] and applied for the imaging of immune cells (phagocytic) and cancer cells (non-phagocytic).

In order to increase the post-vaccination immune response during vaccination of calves against salmonellosis, a test was carried out with an inactivated emulsified vaccine background use probiotic Profort. The studies were on basis large livestock farm in Voronezh region, unfavorable for salmonellosis calves. Epizootological, clinical, immunological, hematological, molecular genetic research me...

Beside its effects on T cells, a direct influence on cells of the myelo-monocytic lineage by GA becomes evident. Recently, we demonstrated that GA drives microglia to adopt properties of type II antigen presenting cells (APC) and increases their phagocytic activity. In the present work, we focused on human blood monocytes in order to examine whether GA may increase phagocytic activity in vivo a...

Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide. To investigate effect silymarin on chronic (COPD). The serum samples 20 healthy controls, patients with acute exacerbation COPD and stable were collected. LPS smoking used to induce mouse model. Our results showed that in COPD, O2-level release from peripheral blood neutrophils negatively correlated forc...

The changes in membrane structure of rabbit polymorphonuclear (PMN) leukocytes during bacterial phagocytosis was investigated with scanning electron microscope (SEM), thin-section, and freeze-fracture techniques. SEM observations of bacterial attachment sites showed the involvement of limited areas of PMN membrane surface (0.01-0.25mum(2)). Frequently, these areas of attachment were located on ...

UNLABELLED Cryptococcosis is a multifaceted fungal infection with variable clinical presentation and outcome. As in many infectious diseases, this variability is commonly assigned to host factors. To investigate whether the diversity of Cryptococcus neoformans clinical (ClinCn) isolates influences the interaction with host cells and the clinical outcome, we developed and validated new quantitat...

BACKGROUND Previous reports have shown that arginine (Arg) enhances phagocytic activity of macrophages and is required for macrophage-mediated toxicity toward tumor cells. Few studies have addressed the importance of Arg supplementation on macrophage and neutrophil function after infection and sepsis. This study examined the effect of Arg-supplemented diets before and Arg-enriched total parente...

Witeska. 1\1.: Changes in Selected Blood Indices of Common Carp ajier Acute Exposure to Cadmium. Acta vet. Bmo 1998.67: 289-293. Haematocrit. red and white blood cell count. phagocytic index. and viability oflymphocytes were studied in common carp exposed for 3 h to 10 mg dm·3 of cadmium. Hematocrit value and RBC count were elevated in all Cd-exposed (n=IO) and control fish (n=IO). compared to ...