Glucose-6-phosphate dehydrogenase (G6PD) deficient subjects are vulnerable to oxidative stress. Quercetin, a flavonoids, has been employed as a potent oxygen-free radical scavenger in order to assess the protective effects of quercetin against H2O2-induced oxidative damage in G6PD-deficient and normal human erythrocytes. Erythrocytes of G6PD-deficient (n = 10) and normal (n = 10) subjects were ...

Anodic memristors obtained by electrochemical anodization of Ta in phosphate, borate and citrate buffer solutions are studied. Memristive behaviour is demonstrated electrical switching between high low conductive states. The endurance retention devices analysed. use phosphate leads to 4 levels the highest ratio resistive All studied oxides stoichiometric Ta2O5 only P detected inside anodic memr...

The purpose of the present study was to evaluate the effect of flueride on resin bonding to artifieially carious enamel. Speeimens frorn demineralized human enamel sections were prepared using two commereially available adhesives (Clearfil SE Bond, Kuraray; Single Bond, 3M} and a composite resin (Clearfil AP-X, Kuraray) according to manufacturers' instructions. They were then immersed in phosph...

the reduction mechanism of molybdenum-methyl thymol blue (mtb)-chlorate system has been investigated in water on a mercury electrode. as this system is very sensitive for ultratrace determination of molybdenum, the actual mechanism involved was studied. electrochemical techniques, including dc tast polarography and cyclic voltammetry were employed. it is shown that, the cathodic wave of molybde...

We examined the mechanism of loss and recovery of steroid binding activity (inactivation and reactivation) of the rat liver glucocorticoid receptor (GR) in the cell free condition. Male Sprague-Dawley rats were adrenectomized 2 days before the hepatectomy for the experiments, and were fed with normal diet and saline. The cytosol was prepared in 10mM phosphate buffer (pH 7.4) containing 10% glyc...

The skeletal isoenzyme of human alkaline phosphatase was partially purified from the serum of a patient with Paget’s disease. The pH dependence of the reaction with p-nitrophenyl phosphate (PNPP) suggests that either the reaction mechanism involves 2 dissociable residues on the enzyme (8.75 > pK1 > 8.2; 9.35 > pKz > 8.75), or that the monoanion is the active form of the phosphoryl substrate. In...

Glucose-6-phosphate dehydrogenase (G6PD) deficient subjects are vulnerable to oxidative stress. Quercetin, a flavonoids, has been employed as a potent oxygen-free radical scavenger in order to assess the protective effects of quercetin against H2O2-induced oxidative damage in G6PD-deficient and normal human erythrocytes. Erythrocytes of G6PD-deficient (n = 10) and normal (n = 10) subjects were ...

A pre-lysis buffer washing procedure was introduced to DNA extraction from a forest soil with high organic matter and iron oxide contents. Sodium phosphate of 0.1 M (pH 7.5) was used as a buffer to wash soil samples when subsequent lysis buffer was phosphate, and 20 mM EDTA (pH 7.5) was used when subsequent lysis buffer included EDTA. Initial experiments were not successful because the DNA extr...