In six normal subjects we investigated the effects of oral phosphatidylcholine (lecithin) on the concentrations of plasma choline, erythrocyte choline, and choline-containing lipids. Plasma choline levels rose 1 hr after treatment and remained elevated for 8 hr, with peaks at 3 and 4 hr after phosphatidylcholine. Erythrocyte choline levels also rose, although the rise was slightly delayed relat...

Abstract The newest method to maintain better bioavailability of antioxidant compounds in the human digestive process is encapsulation using liposomes. liposomes used were lecithin from soybean seeds and L-α-Phosphatidylcholine. Furthermore, curcumin β-carotene encapsulated soy SC-CO2 with 100% ethanol, aquadest 50% v/v ethanol at 50°C, 60°C, 70°C pressure 15 MPa, 20 MPa 25 by ultrasonication f...

INTRODUCTION: Phosphatidylcholine (PC) is a predominant phospholipid present in bile protecting bile ducts from harmful effects of bile acids. In our earlier studies on the analysis bile from cholestatic patients using H MRS, we had observed the hydrolysis of phosphatidylcholine in some patients [1]. Since phosphatidylcholine and its hydrolysis products – glycerophosphocholine (GPC), and phosph...

Extraction with butan-1-ol of freeze-dried microsomal fractions from livers of 3-methyl-cholarthrene-pre-treated hamsters removed about 90% of the total lipid content, but the lipid remaining proved sufficient for the cytochrome P-450 enzyme system to retain about 15-40% of its original catalytic activity for dimethylnitrosamine demethylation. Addition of butan-1-ol-extracted total phospholipid...

New Zealand obese (NZO/HlLt) male mice develop polygenic diabetes and altered phosphatidylcholine metabolism. The gene encoding phosphatidylcholine transfer protein (PC-TP) is sited within the support interval for Nidd3, a recessive NZO-derived locus on Chromosome 11 identified by prior segregation analysis between NZO/HlLt and NON/Lt. Sequence analysis revealed that the NZO-derived PC-TP conta...

Brillouin measurements of the elastic properties are presented for aligned multilamella samples of both pure dipalmitoyl phosphatidylcholine at various temperatures and water concentrations and for the same compound containing 33 mol% cholesterol at various temperatures and two water concentrations. For pure dipalmitoyl phosphatidylcholine the elastic moduli change significantly at the gel tran...

Phosphatidylcholine preparations containing saturated and unsaturated molecular species were subjected to KMnO(4)-NaIO(4) oxidation in aqueous acetic acid, which left only disaturated species intact. After the oxidation, the remaining intact phosphatidylcholine was separated by thin-layer chromatography. The procedure could be used as a simple and rapid method for microdetermination of the satu...

We have developed an improved thin-layer chromatographic method for separation of lung phospholipids. Individual phospholipids are completely separated in the first dimension. All phospholipids, except phosphatidylcholine, are then removed. The phosphatidylcholine-containing area is reacted with osmium tetroxide and saturated phosphatidylcholine species are separated from the unsaturated oxidat...