Transgenic plants are promising alternatives for the low-cost and safe pathogen-free production of complex recombinant pharmaceutical proteins (molecular farming). Plants as higher eukaryotes perform posttranslational modifications similar to those of mammalian cells. However, plant-specific protein N-glycosylation was shown to be immunogenic, a fact that represents a drawback for many plant sy...

One goal of metabolic engineering and synthetic biology for cyanobacteria and microalgae is to engineer strains that can optimally produce biofuels and commodity chemicals. However, the current workflow is slow and labor intensive with respect to assembly of genetic parts and characterization of production yields because of the slow growth rates of these organisms. Here, we review recent progre...

M ixing behavior of three geometrically equivalent 0.06 m bubble column and airlift photobioreactors was compared for use in microalgal culture. Mixing was characterized in terms of the axial dispersion coefficient, mixing time, circulation time and Bodenstein number. Measurements were reported in tap water and sea water, as used in growing microalgal biomass. Compared with the two airlift reac...

An internally illuminated photobioreactor (IIPBR) design is proposed for energy-efficient biomass production. Theoretical rationale of the IIPBR design and its advantages over the traditional bubble column photobioreactors (PBRs) are presented, followed by experimental results from prototype scale cultivation of freshwater and marine algal strains in an 18L IIPBR. Based on theoretical considera...

This study experimentally demonstrated a feed-forward inversion control scheme for maintaining an optimum incident irradiance on photobioreactors (PBRs) during batch cultivation. A data-based modelfree optimization using quadratic fit was utilized to rapidly estimate the optimum average fluence rate set point value that rendered maximum microalgae growth rate. Then, the feed-forward inversion c...

The destruction of mass cultures of microalgae by biological contamination of culture medium is a pervasive and expensive problem, in industry and research. A mathematical model has been formulated that attempts to explain contaminant growth dynamics in closed photobioreactors (PBRs). The model simulates an initial growth phase without PBR dilution, followed by a production phase in which cultu...

Reducing mixing in both flat panel and tubular photobioreactors can result in a positive net energy balance with state-of-the-art technology and Dutch weather conditions. In the tubular photobioreactor, the net energy balance becomes positive at velocities < 0.3 ms-1, at which point the biomass production cost is 3.2 €/kg dry weight. In flat panel reactors, this point is at an air supply rate <...

We recently evaluated the relationship between abiotic environmental stresses and lutein biosynthesis in the green microalga Dunaliella salina and suggested a rational design of stress-driven adaptive evolution experiments for carotenoids production in microalgae. Here, we summarize our recent findings regarding the biotechnological production of carotenoids from microalgae and outline emerging...

The article discusses the potential use of Chlorella microalgae in wastewater treatment plants, at stage disinfection and post-treatment to level corresponding sanitary standards Russian Federation. analysis statistical data related vulgaris suspension as a microbiological chemical element biological before its discharge into water body is carried out. Studied promising areas building energy sa...

The model euryhaline cyanobacterium Synechococcus sp. PCC 7002 has potential as a platform for biotechnological applications because of its genetic amenability and fast growth rates. purpose this work was to measure the outdoor productivity metrics 7002, which we tested by cultivation in 110 L flat-panel photobioreactors 1025 open raceway ponds at Algae Testbed Public Private Partnership (ATP3)...