As an allohexaploid plant, common wheat has a complex gene structure, making it difficult to study its function. Virus-induced silencing (VIGS) is important tool for the rapid analysis of plant In this study, system, namely, barley stripe mosaic virus (BSMV)-VIGS induced by BSMV was used silence phytoene desaturase (TaPDS) and actin depolymerization factor (TaADF7) genes determine effect on whe...

Abstract Background Plants have remarkable diversity in petal colour through the biosynthesis and accumulation of various pigments. To better understand mechanisms regulating pigmentation Lonicera japonica , we used multiple approaches to investigate changes carotenoids, anthocyanins, endogenous hormones gene expression dynamics during transitions, i.e., green bud petals (GB_Pe), white flower (...

Previously, sugarcane mosaic virus (SCMV) was developed as a vector for transient expression of heterologous genes in Zea mays (maize). Here, we show that SCMV can also be applied virus-induced gene silencing (VIGS) endogenous maize genes. Comparison sense and antisense VIGS constructs targeting phytoene desaturase (PDS) showed resulted greater reduction expression. In time course after infecti...

An examination of the carotene fractions extracted from Euglena gracilis Z and pressure-bleached Euglena mutants PR-1, PR-2, PR-3, and PR-4 revealed phytoene in mutants PR-1, PR-2, and PR-3. Photosynthetic E. gracilis Z cultured at different light intensities showed no detectable phytoene, nor was phytoene found in mutant PR-4. However, dark-cultured E. gracilis Z yielded readily assayable amou...

Melon (Cucumis melo L.) is a protected crop in China with high economic value. Agrobacterium-mediated genetic transformation powerful tool to improve agronomic traits and obtain elite germplasm. However, current protocols melons are inefficient highly genotype-dependent. To melon, we tested different infiltration methods for transformation. Among these methods, micro-brushing sonication 20 s, f...

Abstract Genome-editing tools from Oligonucleotide-Directed Mutagenesis (ODM) to CRISPR system use synthetic oligonucleotides for targeted exchange of nucleotides. Presently, majority genome-editing protocols are dependent on the in vitro cell or tissue culture systems with somaclonal variation, and limitations plant regeneration. Therefore, here, we report an alternative planta cellular test o...

To determine whether Saccharomyces cerevisiae can serve as a host for efficient carotenoid and especially beta-carotene production, carotenogenic genes from the carotenoid-producing yeast Xanthophyllomyces dendrorhous were introduced and overexpressed in S. cerevisiae. Because overexpression of these genes from an episomal expression vector resulted in unstable strains, the genes were integrate...

Crocus sativus is a triploid sterile plant characterized by its long red stigmas, which produce and store significant quantities of the apocarotenoids crocetin and crocin, formed from the oxidative cleavage of zeaxanthin. Here, we investigate the accumulation and the molecular mechanisms that regulate the synthesis of these apocarotenoids during stigma development in C. sativus. We cloned the c...