The continuous culture of tomato roots through 1600 passages and for more than 28 years by White (6) indicates the practicability of maintaining plant tissues for very long periods in vitro. This phenomena also supports the theory that under appropriate environmental conditions plant tissues can be maintained in living conditions for an indefinite time. Plant tissues other than roots such as to...

Mass spectrometry imaging (MSI) is a powerful technique for investigating the biomolecular locations within tissues. However, isomeric compounds are rarely distinguished due to inability of MSI differentiate isomers in probing area. Coupling tandem mass with can facilitate differentiating compounds. Here MALDI-TOF/TOF approach was applied spatial distributions disaccharides plant First, MS/MS a...

Plants act as a crucial interface between humans and their environment. The wide use of nanoparticles (NPs) has raised great concerns about their potential impacts on crop health and food safety, leading to an emerging research theme about the interaction between plants and NPs. However, up to this day even the basic issues concerning the eventual fate and characteristics of NPs after internali...

Large-scale single-pass sequencing of cDNAs prepared from specific plant species or tissues has evolved as an inexpensive and efficient gene-discovery tool that can be used to identify novel cDNAs encoding enzymes of specific plant metabolic pathways. Collections of expressed sequence tags from metabolically active tissues can provide quantitative estimates of gene expression levels and thus ar...

a plant viral vector engineered from an in vivo infectious clone of zucchini yellow mosaic virus(zymv) was used to express the human interferon-gamma (inf-γ) in planta. the inf-γ gene was in frame inserted between the p1 and hc-pro orfs of the zymv vector. the infectious activity of the vector was approved by rubbing the plasmid on chenopodium quino a and observing local lesions. individual les...

The discovery of the stimulation of cell division in plant tissues by trace amounts of kinetin, 6(2-furfuryl) aminopurine (11), has resulted in a number of investigations concerned with the effects of this and related compounds on a variety of plant and animal systems (4, 5, 7,9, 10, 12, 13). One system, developed for study of the in situ effect of agents upon the maintenance of normal organiza...