A method that overcomes the difficulties of the 240-nm benzoylcholine method for phenotyping plasma cholinesterases has been developed. After a timed reaction, under the same reaction conditions as in the classic procedure, choline is detected at 500 nm by use of choline oxidase coupled with the peroxidase/phenol/aminoantipyrine system. Cholinesterase activity measurements, calibrated by use of...

We describe a new method for measuring the in vitro rate of hydrolysis of the muscle relaxant succinylcholine. This substrate is hydrolyzed by plasma cholinesterase (EC 3.1.1.8). The resulting choline is determined by measuring the hydrogen peroxide formed on its oxidation by choline oxidase (EC 1.1.3.17). This is done by use of phenol and aminoantipyrine coupled to peroxidase, and yields an in...

background: it is becoming apparent that although inhibition of cholinesterase plays a key role in organophosphate (op) toxicity, other factors are also important. one of the contributing factors for severity of op poisoning is electrolyte imbalances such as hypokalemia. this study was aimed at evaluating the value of hypokalemia in association with plasma cholinesterase (pche) levels in predic...

OBJECTIVES This study presents criteria for assessing the individual pesticide burden of workers in the chemical industry. METHODS A group of 1003 workers exposed to methylparathion or ethylparathion (alkyl phosphates), propoxur (carbamate), or cyfluthrin (pyrethroid) was investigated. After exposure to methylparathion or ethylparathion the methylparathion or ethylparathion and methylparaoxon...