We report on a novel approach for the size-dependent fractionation of protein assemblies on polymeric surfaces. Using a simple temperature gradient method to generate one-dimensional gradients of grafted poly(ethylene glycol), we fabricated silicon-oxide chips with a gradually changing surface density of kinesin motor molecules. We demonstrate that such a bioactive surface can be used to sort g...

The use of proteins as therapeutics has a long history and is becoming ever more common in modern medicine. While the number of protein-based drugs is growing every year, significant problems still remain with their use. Among these problems are rapid degradation and excretion from patients, thus requiring frequent dosing, which in turn increases the chances for an immunological response as wel...

Studyon cultivation of Polyporus squamosusin aqueoustwo-phase systemconsisting of7% (wi w) polyethylene glycol (M" 4,000) and 7.5% dextran (M" 40,000) wasconducted. Fungusgrowth wasrestricted in bottom phase, while thepresence of endo-pectinase activity in one of thephases waschangeable duringthecultivation. All endo-pectinase activity excreted on the 3,d dayofcultivation wasseparated in the to...

The mechanochemical preparation of highly functionalized 3,5-disubstituted hydantoins was investigated in the presence of various poly(ethylene) glycols (PEGs), as safe grinding assisting agents (liquid-assisted grinding, LAG). A comparative study under dry-grinding conditions was also performed. The results showed that the cyclization reaction was influenced by the amount of the PEG grinding a...

We report a novel simple one-pot strategy for fabricating pure and highly planar silver (Ag) crystal belts. Unique single-crystal Ag belts (high width-to-thickness ratio ~50) were successfully synthesized in high yield (80 wt%) by reducing AgNO3 using N,N,N',N'-tetramethylethylenediamine (TEMED) as a reducing and a structure-determining agent in the presence of polyethylene glycol (PEG) under m...

DNA strand exchange is catalyzed by molecular crowding and hydrophobic interactions in concentrated aqueous solutions of polyethylene glycol, a discovery of relevance for understanding the function of recombination enzymes and with potential applications to DNA nanotechnology.

Novel first (G1) and second (G2) generation dendrimers based on three-fold branched oligoethylene glycol dendrons are efficiently synthesized which show characteristic thermoresponsive behavior and negligible cytotoxicity (for G2).

Microgel particles capable of bulk degradation have been synthesized from a solution of diacrylated triblock copolymer composed of poly(ethylene glycol) and poly(lactic acid) in a microfluidic device using stop-flow lithography (SFL). It has been previously demonstrated that SFL can be used to fabricate particles with precise control over particle size and shape. Here, we have fabricated hydrog...

Crystals of a designed six-finger zinc-finger protein, Aart, bound to a 22-base-pair duplex DNA containing a consensus binding site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as the precipitating agent. The irregularly shaped crystals belong to space group P1, with unit-cell parameters a = 41.95, b = 71.76, c = 74.73 A, a...