the protective effects of rheum tanguticum polysaccharide 1 (rtp1), which is extracted from the chinese traditional medicine rheum tanguticum, on radiation-induced intestinal mucosal injury was investigated. rat intestinal crypt epithelial cells (iec-6 cells) and sprague-dawley rats were each divided into control, irradiated and rtp1-pretreated irradiated groups. after irradiation, cell surviva...

The anomeric proton magnetic resonances of Mycobacterium smegmatis 3-O-methylmannose polysaccharide have chemical shifts intermediate betwen those of nonaglucoamylose and alpha-cyclodextrin, and on addition of palmitic acid most of these resonances are shifted upfield toward that of the cyclodextrin. This suggests that the methylated polysaccharide could have a conformation with some secondary ...

A sulfated polysaccharide extracted from Navicula sp. presented a yield of 4.4 (% w/w dry biomass basis). Analysis of the polysaccharide using gas chromatography showed that this polysaccharide contained glucose (29%), galactose (21%), rhamnose (10%), xylose (5%) and mannose (4%). This polysaccharide presented an average molecular weight of 107 kDa. Scanning electron microscopy (SEM) micrograph...

The accumulation of sulfate-(35)S by Porphyridium aerugineum cells and subsequent appearance of solubilized capsular polysaccharide-(35)S in the growth medium were examined The uptake of label by the cells was largely light dependent. Pulse-chase experiments using log phase cells revealed a rapid labeling of solubilized capsular polysaccharide, recovered from the medium as the cetylpyridinium c...

The polysaccharide from cell walls of coagulase-positive staphylococci coated both latex particles and tanned red cells for agglutination by human sera and by specific staphylococcal antisera. Treatment with trypsin or autoclaving destroyed the capacity of polysaccharide to coat particles but did not affect precipitation of antibody. Periodic acid destroyed both properties. The teichoic acid po...

The type V capsular polysaccharide of group B Streptococcus has been isolated and purified, and its repeating unit structure determined. The native type V polysaccharide contains D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, and sialic acid in a molar ratio of 3:2:1:1. Methylation analysis and 1H NMR and 13C NMR analysis of the native type V polysaccharide and of its specifically degra...

The anomeric proton magnetic resonances of Mycobacterium smegmatis 3-0-methylmannose polysaccharide have chemical shifts intermediate between those of nonaglucoamylose and cu-cyclodextrin, and on addition of palmitic acid most of these resonances are shifted upfield toward that of the cyclodextrin. This suggests that the methylated polysaccharide could have a conformation with some secondary st...

Extracts of defatted cocoa inhibited the biosynthesis of extracellular polysaccharide by both cell-free and cell-associated streptococcal glucosyltransferases, but did not affect growth or acid production. Both water-insoluble and water-soluble polysaccharide syntheses were inhibited, and the net result was a shift away from adherence-supportive polysaccharide. Inhibition was non-competitive wi...

in recent years there has been considerable interest in the synthesis and separation of enantiomers of organic compounds especially because of their importance in the biochemistry and pharmaceutical industry. high-performance liquid chromatography is a very useful method for the direct separation of enantiomers. however, about 30−40 years ago, commercially available chiral stationary phases wer...

Four seaweed polysaccharides were extracted from Sarcodia ceylonensis, Ulva lactuca L., Gracilaria lemaneiformis, and Durvillaea antarctica, respectively, by microwave-assisted extraction. The effect of three significant variables (extraction time, extraction temperature, and the ratio of water to raw material) on the process for extracting polysaccharides was investigated, along with the optim...