Traditional Chinese medicinal herbs containing berberine have been historically used to prevent miscarriage. Here, we investigated whether the anti-apoptotic effects of berberine on pre-implantation embryonic development are regulated by miRNA-21. Mouse pronuclear embryos were cultured in medium with or without berberine, and some were then microinjected with a miRNA-21 inhibitor. The in vitro ...

Implantation failure after IVF is one of the factors associated with a reduced chance of pregnancy for some patients. Assisted hatching methodologies are designed to facilitate the embryo's escape from the zona pellucida, and this strategy has been suggested as a means of improving pregnancy rates in patients with previous implantation failure. The aim of this prospective and randomized study w...

oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. progesterone (p4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

BACKGROUND The high number of IVF procedures performed in Israel has had an unforeseen consequence: accumulation of large amounts of surplus frozen embryos. After five years that the frozen embryos are kept for free, patients need to make an embryo disposition decision. One option is donation for research. The donation rate in Israel is very low. Our aim was to understand the attitudes, values ...

objective(s): progestrone is a prequisite for pre-implantation angiogenesis and induce decidual angiogenesis. it is unknown the effect of progestrone administration on the endometrium of hyperstimulated mice at pre-implantation time. material and methods: adult female nmri mice were divided in three groups [control group, ovarian stimulated group and progestrone treated mice after ovarian stimu...

s:1454:"Background: Intracytoplasmic sperm injection(ICSI) outcome is tighly depending of male and female factors. The assessment of several clinical and laboratory parameters may predict results of ICSI. Materials and Methods: We restrospectively analysed patients who had ICSI for male or female factor infertility. The clinical and laboratory factors that influenced the fertilization, pregnanc...

Graphical Abstract Highlights d Reversion or derivation of hESCs in 5iLAF results in SSEA4-negative cells d SSEA4-negative hESCs show gene expression consistent with naive pluripotency d Naive hESCs show lost ''memory'' of gamete and blastocyst methylation d Imprinting is lost in naive hESCs In Brief Pastor and colleagues show that reversion of primed hESCs in 5iLAF, or derivation of hESCs in 5...

BACKGROUND The purpose of this study was to evaluate the respective influences of blastomere survival and resumption of mitosis on the outcome of frozen-thawed embryos. METHODS A retrospective analysis was performed in our centre on 363 thawing cycles, involving 4-cell day 2 grade 1 embryos with <10% fragmentation. RESULTS A higher implantation rate per transferred embryo was observed when ...

The renin-angiotensin system (RAS), mainly associated with the regulation of blood pressure, has been recently investigated in female reproductive organs and the developing foetus. Angiotensin II (Ang II) influences oviductal gamete movements and foetal development, but there is no information about RAS in the early embryo. The aim of this study was to determine whether RAS components are prese...