We investigated the efficacy and utility of a post-column derivatization method for the detection of amines with o-phthalaldehyde (OPA) in ultra high-performance liquid chromatography (UHPLC). Since it was anticipated that a derivatization reaction system placed downstream of the column would give considerable band broadening to narrow peaks generated by a UHPLC column, we examined the contribu...

During the past decade, technological innovations based on new knowledge of the principles underlying separations processes have transformed column liquid chromatography, the oldest chromatographic technique, into a sophisticated tool for rapidly achieving the most difficult of separations and analyses. Applications of this modern version called High Performance Liquid Chromatography (HPLC) are...

The cyclic voltammetric behavior of acetaldehyde and the derivatized product with 2,4-dinitrophenylhydrazine (DNPHi) has been studied at a glassy carbon electrode. This study was used to optimize the best experimental conditions for its determination by high-performance liquid chromatographic (HPLC) separation coupled with electrochemical detection. The acetaldehyde-2,4-dinitrophenylhydrazone (...

During the search for neuraminidase inhibitors from medicinal fungi, we found that the culture broth of Phellinus linteus exhibited potent inhibitory activity. Solvent partition, Sephadex LH-20 column chromatography, and high-performance liquid chromatography (HPLC) were performed for purification of two active substances from the culture broth. According to (1)H NMR measurements and comparison...

The current trend in liquid chromatography is towards the achievement of higher kinetic efficiency and shorter analysis time. Different types of column packings are now available for attaining very fast and high resolution separations without changing instruments, worrying about high backpressure or compromising column longevity. In recent developments of particle technology, the use of superfi...

Abbreviations and trivial names: chenodeoxycholate, 3a,7adihydroxy-5p-cholanate; TLC, thin-layer chromatography; PLC, preparative-layer chromatography; HPLC, high-pressure liquid chromatography; PMR, proton magnetic resonance spectroscopy; GLC, gas-liquid chromatography; MS, mass spectrometry. Address correspondence to William H. Elliott, E. A. Doisy Department of Biochemistry, St. Louis Univer...

background aflatoxins are a group of very toxic compounds produced by a fungal species and are found in food products. aflatoxin m1 (afm1) is a type found in dairy products and resistant to pasteurization. afm1 can adversely affect hepatocytes in human and leading to various liver diseases. thus, it is essential to examine raw milk for the presence of afm1. conclusions according to the results ...

Drug counterfeiting has exaggerated dramatically over the past decade causing a significant threat to human health. It must be processed in regard to medicines and the matter isn't restricted to a violation of belongings rights, however clearly involves every kind of poor quality pharmaceutical merchandise, including falsified and substandard medicine. The present paper discuss the review of th...

Primaquine, an 8-amino-6-methoxyquinoline antimalarial agent, was subjected to metabolic studies with microorganisms. Streptomyces rimosus converted primaquine to the previously reported N-acetyl derivative. Continued incubation of S. rimosus resulted in the formation of a minor dimeric metabolite. The structure of the minor dimeric metabolite was proposed based primarily on its spectral data (...

An acid extract of pedal ganglia of the mollusc Mytilus edulis was fractionated by high-pressure liquid chromatography with a reverse-phase column. Peak fractions with retention times of those of [Met]- and [Leu]enkephalin were subjected to binding assays in both invertebrate and vertebrate tissues. The results showed that these fractions have the same binding activities as authentic enkephalin...