Background and objectives: Alzheimer's disease (AD) as a neurodegenerative disorder is the most common form of dementia in the elderly. According to the amyloid hypothesis, accumulation of amyloid beta (Aβ) plaques, which are mostly constituted of Aβ peptide aggregates, triggers pathological cascades that lead to neuronal cell death. Thus, modulation of Aβ toxicity is the hopef...

PURPOSE The goal of the present study was to establish a protocol for primary culture of lacrimal gland acinar cells (LGACs) and to assess the effect of adding insulin to the culture media. METHODS LGACs were isolated and cultured from lacrimal glands of Wistar male rats. The study outcomes included cell number, viability, and peroxidase release over time and in response to three concentratio...

Wann-Yee Her, Mei-Fang Yang, Tin-Hsin Hsiao and Keh-Min Liu (1994) Morphological studies of cardiac ganglionic neurons in primary cell culture. Zoological Studies 33(2): 108-113. In mammals, several groups of cardiac ganglia (CG) are sparsely distributed in the posterior wall of the atrium and the interatrial septum. The CG from early postnatal rats were cultured for optimum observation of cell...

The primary culture bone marrow (PCBM) cells are often used in different cytological investigations. Here we study the behavior of porcine unstimulated PCBM cells during the cultivation. DNA cytophotometry analyses revealed that immature white blood cells (WBC) such as lymphoblasts, monoblasts and myeloid cells during cultivation process reduced the DNA content in the nuclei. This resulted in d...

Long-term survival in lung transplantation is limited by the development of obliterative bronchiolitis, a condition characterised by inflammation, epithelial injury, fibroproliferation and obliteration of bronchioles leading to airflow obstruction. To investigate the role of the bronchial epithelium in the pathogenesis of obliterative bronchiolitis the current study aimed to establish primary b...

Tissue culture has been used for the study of animal viruses since 1906 when Aldershoff and Boers first grew vaccinia in rabbit tissue separate from the whole host.2 Other limited successes were obtained in the years following, but extensive use of tissue culture in animal virology awaited the studies of Enders, Weller, and Robbins' on a human agent, poliovirus. Since then, the use of tissue cu...