Neuroserpin, a member of the Serine Proteinase Inhibitor (Serpin) superfamily, is known to be a neuroprotective factor in the focal ischemic stroke followed by reducing the microglial activation. Neuroserpin is a protein rich of methionine residues that can scavenge the free radical species which may increase its neuroprotective effect. On the other hand, the oxidative modifications of the amin...

The selectivity of serine proteinases action is mediated by high-specifice binding the proper parts protein substrate. Among such targets a special place belongs to areas functionally conditioned interaction with active center enzyme. Their sharp difference in enzyme affinity due synchronous and allosteric sites site specific amino acid residues substrate that are adequate specificity placed co...

The kinetics of ligand binding to the periplasmic C4-dicarboxylate binding protein (DctP) from Rhodobacter capsulatus were investigated by exploiting the changes in the intrinsic fluorescence of the protein upon binding ligands. Steady state measurements have shown that L-malate, succinate, and fumarate are all bound with sub-micromolar Kd values, whereas D-malate is bound 2 orders of magnitude...

• The Writhe and Torsion are used to define a local topological free energy of proteins. High conformations independent sequence. secondary structure. may be related the rate limiting step in protein folding. Protein folding, process by which proteins attain 3-dimensional conformation necessary for their function, remains an important unsolved problem biology. A major gap our understanding is h...

Specific markers for diseases associated with protein aggregate depositions are of great interest. Here we report the use of conjugated polyelectrolytes as conformation-sensitive optical probes for histological labeling of amyloid deposits in ex vivo tissue samples-amyloid light chains in primary systemic amyloidosis, islet amyloid polypeptide in human pancreas, and Abeta amyloid in Alzheimer's...

introduction: the unprotected eye is extremely sensitive to laser radiation and can be permanently damaged from direct or reflected beams. the area of the eye damaged by laser energy is dependent upon the wavelength of the incident laser beam, duration of exposure and tissue characteristics. this study aims to investigate the effect of intense green light from doubled frequency neodymium-doped ...

G protein-coupled receptor (GPCR) kinases (GRKs) phosphorylate activated GPCRs, priming them for arrestin binding and internalization. How GRKs recognize these receptors is unknown, but most studies indicate that a conserved region at their extreme N-terminus key. Herein we report the cryo-EM structure of light rhodopsin (Rho∗) in complex with kinase (GRK1) along supporting functional confirm k...

SCit is a web server providing services for protein side chain conformation analysis and side chain positioning. Specific services use the dependence of the side chain conformations on the local backbone conformation, which is described using a structural alphabet that describes the conformation of fragments of four-residue length in a limited library of structural prototypes. Based on this con...