PURPOSE To determine anti-HCV antibodies and genomic subtype of HCV in 1487 confirmed human immunodeficiency virus (HIV) positive samples. METHODS A total of 1487 confirmed HIV-positive samples were tested for anti-HCV antibodies by using a third generation ELISA kit (Ortho 3.0) and by RT PCR for HCV. HIV and HCV coinfected samples were selected for HCV genotyping by RFLP and subtyping with N...

abstract the present research was conducted in a commercial orchard of pistachio during 2006-2007 in rafsanjan, iran. gibberellic acid (ga3) were applied at 0, 25, 50 and 75 mgl-1 and at three time of spray (the first of june, july and august) on “ahmad-aghai” pistachio cultivar. two branches of each tree was sprayed during two years of study. spraying at june had least of early splitting. ga3 ...

Five methods using aqueous/organic solvents for the separation of proteins from oils were compared. The extraction with acetone-hexane followed by amino acid analysis was found to be the most suitable method for isolation and quantification of proteins from oils. The detection limit of the method was 0.18 mg protein/kg oil, and the quantification limit was 0.6 mg protein/kg. The relative repeat...

The protein content of dry biomass of the microalgae Porphyridium cruentum, Scenedesmus almeriensis, and Muriellopsis sp. and of the cyanobacteria Synechocystis aquatilis and Arthrospira platensis was measured by the Lowry method following disruption of the cells by milling with inert ceramic particles. The measurements were compared with the Kjeldahl method and by elemental analysis. The nitro...

Total lipids, fatty acid composition and heavy metal content of Sardina pilchardus fillet samples captured in February 2014 in Beni saf, Mostaganem, Ghazaouet, Algiers and Jijet coast were evaluated. Total lipid content was related to the five sites of catch (p < /em>< 0.05). Concerning the heavy metals, the levels of lead (Pb) ranged from 0.013mg to 0.024mg, however those of mercury (Hg) varie...

A thermoplastically extruded snack was produced from different blends of corn grits and silver carp minced meat. Response Surface Methodology (RSM) was used to study the effect of fish meat content and to optimize the feed composition. Fourteen treatments from central composite design considering minced fish content (0-3 kg) and corn (7-10 kg) were used as the independent variables. The expansi...

The proximate composition and various specific components of Antarctic krill Euphausia superba, in the catch season between March and August were investigated. Frozen krill were freeze-dried and milled. The proximate composition comprised water, proteins, fats, ash, fatty acids, and amino acids, while the specific components were vitamins, minerals, nucleotides, betaine, and astaxanthin. The mo...

background: sand fly saliva helps parasite establishment and induce immune responses in vertebrate hosts. in the current study, we investigated the modulation of phlebotomus papatasi salivary gland antigen expression by sea­sonal and biological factors. methods: sand flies were grouped according to physiological stages such as unfed, fed, semi-gravid, gravid, parous, nulliparous, infected or no...