نتایج جستجو برای: proteinase k
تعداد نتایج: 387308 فیلتر نتایج به سال:
BACKGROUNDS Shigellosis remains an important public health problem in developing countries with S. sonnei and S. flexneri in US, Europe and in Asian countries being of importance. OBJECTIVES This study evaluates the protective effect of Lactobacillus casei cell-free culture supernatants (CFCS) against multiple drug resistance (MDR) clinical samples of Shigella sonnei and Shigella flexneri in ...
Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render microfocus beamlines at synchrotron-radiation sources suitable for room-temperature serial crystallography data collection also. Owing to the ...
For the first time, it is demonstrated that exposure of an enzyme to anhydrous organic solvents at optimized high temperature enhances its catalytic power through local changes at the binding region. Six enzymes, namely, proteinase K, wheat germ acid phosphatase, alpha-amylase, beta-glucosidase, chymotrypsin and trypsin were exposed to acetonitrile at 70 degrees C for three hr. The activities o...
proteinase 3(pr3) is a human polymorphonuclear leukocyte serine proteinase and is the main target antigen for antineutrophil cytoplasmic antibodies (anca) found in wegener's granulomatosis (wg). we developed a stable expression system for conformationally intact recombinant pr3 (rpr3) in chinese hamster ovary cells (cho-cells). the part of pr3 cdna that encoded the active form of pr3 was s...
Nosocomial infection caused by methicillin-resistant staphylococci poses a serious problem in many countries. The aim of this study was to rapidly and reliably detect methicillin-resistant-staphylococci in order to suggest appropriate therapy. The presence or absence of the methicillin-resistance gene in 115 clinical isolates of Staphylococcus aureus and 50 isolates of Coagulase Negative Staphy...
Agarose gels provide the ideal environment for studying nucleation step of complex biomacromolecules under diffusion-controlled conditions. In present paper, we characterized influence agarose on three model proteins, i.e., lysozyme, insulin, and proteinase K, as a function concentration using batch method set-up inside flat capillaries. By this set-up, were able to directly count number crysta...
Proteinase A and proteinase B, two vacuolar enzymes in Saccharomyces cerevisiae, are synthesized as larger precursors with apparent molecular weights of approximately 52,000 and 42,000, respectively. These precursor molecules are processed to their mature forms of 42,000 molecular weight for proteinase A and 33,000 molecular weight for proteinase B. In the presence of tunicamycin, an inhibitor ...
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