Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5)/g mycelia and 2.8x10(5)/g mycelia were formed in strains A and R respectively. Osmotic stabilizer sucrose 1M gave ma...

A heterotrophic organism 1-2 billion years ago enslaved a cyanobacterium to become the first photosynthetic eukaryote, and has diverged globally. The primary phototrophs, glaucophytes, are thought to retain ancestral features of the first photosynthetic eukaryote, but examining the protoplast ultrastructure has previously been problematic in the coccoid glaucophyte Glaucocystis due to its thick...

Cota-Robles, Eugene H. (University of California, Riverside). Electron microscopy of plasmolysis in Escherichia coli. J. Bacteriol. 85:499-503. 1963.-Escherichia coli cells plasmolyzed in 0.35 m sucrose reveal plasmolysis at one tip of a cell or in the center of dividing cells in which protoplast partition has been complete. Central plasmolysis reveals that protoplast separation can be complete...

This research is aimed to improve the oxytetracycline production from Streptomyces rimosus. Five relatively high oxytetracycline yielding strains were obtained by nitrosoguanidine mutation of the protoplast of initial oxytetracycline producing strain Streptomyces rimosus JH and then subjected to genome shuffling as parental strains. The protoplasts of the five parental strains were prepared and...

Although we have made great progress in developing resistance to root – knot nematodes in cucumber, no source has been created or screened to resistance to M. incognita, the main species affecting cucumber (9, 10). Resistance to M. incognita exists in horned cucumber (Cucumis metuliferus), but efforts by many research groups (including our own) to make crosses of Cucumis species with cucumber h...