A series of novel aromatic carboxylic acid amides were synthesized and tested for their activities against six phytopathogenic fungi by an in vitro mycelia growth inhibition assay. Most of them displayed moderate to good activity. Among them N-(2-(1H-indazol-1-yl)phenyl)-2-(trifluoromethyl)benzamide (3c) exhibited the highest antifungal activity against Pythium aphanidermatum (EC50 = 16.75 µg/m...

An improved protocol for the isolation of high-quality DNA from fungi is described. The method involves inactivating proteins by SDS/proteinase K and precipitating polysaccharides in the presence of high salt. Further purification is based on differential solubility of DNA and high molecular weight polysaccharides in aqueous media. The procedure does not use the toxic and potentially hazardous ...

Cinnamic, p-coumaric and ferulic acids were isolated from pineapple stems (Ananas comosus var. Cayenne). Twenty-four kinds of esters were prepared from these acids, alcohols and the components of Alpinia. Isopropyl 4-hydroxycinnamate (11) and butyl 4-hydroxycinnamate (12) were found to have almost the same effectiveness in antifungal activity against Pythium sp. at 10 ppm as that of the commerc...

greenhouse-grown red pine seedling, Pinus resinosa, when cultured on malt agar or potato dextrose agar (PDA) showed inhibitory activity against selected test fungi. Sensitive fungi included Ceratocystis ulmi (BUISM.) C. MOREAU, Pythium spp., Rhizoctonia solani KUHN, Cylindrocarpon sp., Fusarium oxysporum SCHLECT., and Sirococcus strobilinus PREUSS. When grown on PDA, a colorless crystalline sol...

Two independent experiments were conducted to investigate the influence of rhizosphere bacteria on the growth of Anthoxanthum odoratum and Panicum sphaerocarpon. We tested whether host-specific populations of Bacillus mycoides affected the growth of their Anthoxanthum and Panicum hosts and whether host-specific differences in Bacillus populations modified the strong detrimental effect of the ro...

we assessed a collection of 47 fluorescent pseudomonas spp., some with known biological     control activity against certain soil-borne phytopathogenic fungi such as, macrophomina phaseolina, rhizoctonia solani, phytophthora nicotianae var. parasitica, pythium sp. and  fusarium sp. in vitro and the potential to produce known secondary metabolites such as, siderophore, hcn and protease. the resu...