The reading frame of the HIV-1 pol gene, encoding protease (PR) and reverse transcriptase (RT), including RNase H as well as integrase, was fused to the bacterialbeta-galactosidase gene and overexpressed in Escherichia coli cells. The resulting fusion protein was cleaved autocatalytically leading to PR, RT and integrase. Immunoprecipitations of bacterial crude extracts with anti-RT antibodies p...

This study is a linear stability analysis of the flows induced by ultrasound acoustic waves (Eckart streaming) within an infinite horizontal fluid layer heated from below. We first investigate the dependence of the instability threshold on the normalized acoustic beam width H(b) for an isothermal fluid layer. The critical curve, given by the critical values of the acoustic streaming parameter, ...

Treatment of toluene solutions of the silylenes Si(SAr(Me6))2 (Ar(Me6) = C6H3-2,6(C6H2-2,4,6-Me3)2, 1) or Si(SAr(Pr(i)4))2 (Ar(Pr(i)4) = C6H3-2,6(C6H3-2,6-Pr(i)2)2, 2) with excess ethylene gas affords the siliranes (Ar(Me6)S)2SiCH2CH2 (3) or (Ar(Pr(i)4)S)2SiCH2CH2 (4). Silirane 4 evolves ethylene spontaneously at room temperature in toluene solution. A Van't Hoff analysis by variable-temperatur...

Federal regulations require that refuge alternatives (RAs) be located within 305 m (1,000 ft) of the working face and spaced at one-hour travel distances in the outby area in underground coal mines, in the event that miners cannot escape during a disaster. The Mine Safety and Health Administration mandates that RAs provide safe shelter and livable conditions for a minimum of 96 hours while main...

We use an analytic toy model of turbulent convection to show that most of the scaling regimes are spoiled by logarithmic corrections, in a way consistent with the most accurate experimental measurements available nowadays. This sets a need for the search of new measurable quantities which are less prone to dimensional theories. 47.27 -i Turbulent flows, convection and heat transfer 47.27.Eq Tur...

A theoretical analysis of the influence temperature and nanoparticle concentration on free convection heat transfer from a horizontal tube immersed in an unbounded nanofluid was presented. The Nusselt (Nu) number coefficient were parameters intensity convective transfer. For convection, Nu function Rayleigh (Ra) Prandtl (Pr) number. functions thermophysical properties nanofluids. nanofluids var...

We have undertaken a systematic study of thermal conductivity as a function of temperature and magnetic field of single crystals of the compound (La12zPrz)1.2Sr1.8Mn2O7 for z(Pr)50.2,0.4, and 0.6. The lattice distortion due to Pr substitution and anisotropic thermal conductivity in bilayer manganites are discussed on the basis of different relaxation models of local lattice distortions in metal...

Antineutrophil cytoplasmic antibodies (ANCAs) against myeloperoxidase (MPO), proteinase 3 (PR-3), lactoferrin (LF), cathepsin G (CG) and elastase (EL) were determined to investigate whether the presence of ANCAs is closely related to extra-articular manifestations in Japanese patients with rheumatoid arthritis (RA). Antibodies against MPO, PR-3, LF, CG and EL were determined in sera from 125 pa...

We study the influence of thermal boundary conditions on large aspect ratio Rayleigh-Bénard convection by a joint analysis experimental and numerical data sets for Prandtl number Pr=7 Rayleigh numbers Ra=105−106. The spatio-temporal are obtained combined Particle Image Velocimetry Thermometry measurements in cuboid cell filled with water at an Γ=25. In addition, generated Direct Numerical Simul...

Mine Safety and Health Administration (MSHA) regulations require underground coal mines to use refuge alternatives (RAs) to provide a breathable air environment for 96 hrs. One of the main concerns with the use of mobile RAs is the heat and humidity buildup inside the RA. The accumulation of heat and humidity can result in miners suffering heat stress or even death. To investigate this issue, t...