Purpose Radiolabeled antibodies and peptides hold promise for molecular radiotherapy but are often limited by a low payload resulting in inadequate delivery of radioactivity to tumour tissue and, therefore, modest therapeutic effect. We developed a facile synthetic method of radiolabeling indium-111 (111In) to epidermal growth factor (EGF)-gold nanoparticles (111In-EGF-Au NP) with a high payloa...

Amyloid is a complex pathologic matrix comprised principally of paracrystalline protein fibrils and heparan sulfate proteoglycans. Systemic amyloid diseases are rare, thus, routine diagnosis is often challenging. The glycosaminoglycans ubiquitously present in amyloid deposits are biochemically and electrochemically distinct from those found in the healthy tissues due to the high degree of sulfa...

Peptide receptor radionuclide therapy (PRRT) has been shown to be an effective treatment for neuroendocrine tumors (NETs) if curative surgery is not an option. A majority of NETs abundantly express somatostatin receptors. Consequently, following administration of somatostatin (SST) analogs labeled with γ-emitting radionuclides, these tumors can be imaged for diagnosis, staging or follow-up purp...

A dodecapeptide EDIKPKTSLAFR ligand targeting CEN-1 human nasopharyngeal carcinoma (NPC) was identified by in vivo phage display. Two tridecapeptides and their derivatives, named YR13 (YEDIKPKTSLAFR), EY13 (EDIKPKTSLAFRY), EY13-NH2 (EDIKPKTSLAFRY-NH2) and Fmoc-YR13 (Fmoc-YEDIKPKTSLAFR), were synthesized and radiolabeled with 131I. The stability in vitro, biodistribution and tissue distribution ...

The often fatal outcome of ovarian cancer (OC) is related to inadequate detection methods, which may be overcome by development of nuclear imaging agents. Cancer targeting peptides have been identified using in vivo bacteriophage (phage) display technology; however, the majority of these ligands target tumor vasculature. To overcome this problem, a two-tier phage display method was employed to ...

The natriuretic peptides are considered to be cardioprotective; however, their receptors have not been identified in human myocardium using radiolabeled analogs. Dendroaspis natriuretic peptide (DNP) has been recently identified as a new member of this peptide family and is thought to be less susceptible to enzymatic degradation. Therefore, we have developed the novel radiolabeled analog [125I]...

pr81 is a monoclonal antibody that binds with high affinity to muc1 that over expressed on breast tumors. pr81 is considered a suitable targeting molecule that was radiolabeled using cu-64 for positron imaging studies. the monoclonal antibody was conjugated with dota moiety and after purification was evaluated for radiochemical purity, immunoreactivity, cell toxicity and structure integrity as ...

INTRODUCTION The integration of diagnostic testing for the presence of a molecular target is of interest to predict successful targeted radionuclide therapy (TRNT). This so-called 'theranostic' approach aims to improve personalized treatment based on the molecular characteristics of cancer cells. Moreover, it offers new insights in predicting adverse effects and provides appropriate tools to mo...

This study examines the clearance and early hydrolysis of atrial natriuretic factor (ANF) in vivo. Radiolabeled ANF was cleared from the circulation of the rat with biphasic kinetics; the majority (90%) of ANF cleared with a t1/2 of 15 s, the remaining peptide was cleared with a t1/2 of 5 min. Microsequence analysis of ANF peptides recovered from the circulation of rats revealed five major degr...

introduction: somatostatin receptors expressed on a wide range of human tumors, are potential targets for the peptide receptor radionuclide therapy (prrt). in this study, 177lu-[dota-dphe1, tyr3]octreotide (177lu-dotatoc) as an agent for prrt was prepared and its biodistribution was studied in rats. methods:the best condition for the preparation of the 177lu-dotatoc radiolabeled complex was det...