Objective: The study aimed to compare intracytoplasmic sperm injection (ICSI) outcome of patients with epididymal sperm and testicular sperm in different paternal age with obstructive azoospermia. Methods: We retrospectively studied the records of 177 men with obstructive azoospermia who underwent sperm retrieval for ICSI. 71 cases were performed with testicular sperm aspiration (TESA), 106 cas...

this study investigated the concentration/time dependent effects of nanosilver colloid on the motionparameters of the ejaculated ram spermatozoa in vitro. after incubation of sperm samples for 30, 60, 120 and 180 min in the presence of nanosilver colloid (0, 0.001, 0.01, 0.1, 1 and 10 ppm), the motion parameters were evaluated by computer assisted sperm analysis. there was a significant (p

Epidermal growth factor (EGF) is one of the important cytokines that play a role in fertility. It is known that EGF affects both male and female reproduction, but its effect on sperm parameters is not fully understood. Up to the present, the effect of EGF on ram sperm motility parameters has not been published. We analyzed motility parameters of ejaculates after 24, 48, and 72 hours from the EG...

This work was designed to study how this ability is affected by different sperm treatments routinely used for in vitro fertilization (IVF) assay. In this study, boar sperm samples from epididymal or ejaculated origin were processed by three different methods: left unwashed (NW group), washed in Dulbecco's phosphate-buffered saline supplemented with 0.1% BSA (BSA group), and washed on a Percoll(...

Background: Aluminum, as an environmental pollutant, can exert oxidative stress on spermatogenesis and sperm morphology. Silymarin, an effective substance extracted from Silybum marianum, is a potent antioxidant which inhibits oxidative stress. Due to the damaging effects of aluminum on the male reproductive system as well as sperm and the role of silymarin, this study was performed to investig...

We observed the influences of low-temperature storage of the feline epididymis on the epididymal semen qualities before and after cryopreservation to identify the optimal duration for low-temperature storage of the epididymis. After excision, the feline epididymis was stored at 4 degrees C for 0-72 hr and then subjected to epididymal sperm collection. When sperm from the refrigerated cauda epid...

This study was done to evaluate the effect of sperm source on chromatin integrity and ICSI outcomes. One hundred and thirteen samples containing epididymal aspirates of 57 obstructive azoospermic men and 56-ejaculated semen of normozoospermic men were included in this study. Sperm chromatin status was evaluated by Chromomycin A3 (CMA3), Aniline Blue (AB) and Toluidine Blue (TB). Fertilization r...

The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epid...

Background: Epididymal spermatozoa have been used for production of offspring from genetically important animals after they are dead. To attain optimal fertility with epididymal spermatozoa, they should be preserved in a suitable medium in 37°C. Preservation media provides requirement for spermatozoa and affects its motility, viability, and fertility. Sucrose is one of the important components ...