In the process of cryopreservation, semen farm animals can be threatened by many factors, such as oxidative damage, which affect motility and plasma membrane function sperm. As an endogenous antioxidant in animals, glutathione (GSH) significantly improve quality thawed sperm when added to diluent semen. This study was aimed at examining effect GSH (given 0.5 mM, 1.5 mM 5 mM) on viability ram fo...

Background: Basal level of reactive oxygen species (ROS) is essential for sperm acrosomal reaction, hyperactivation, motility, capacitation and fertilization. Ram spermatozoa contain large quantities of polyunsaturated fatty acids in their plasma membranes so are extremely susceptible to ROS induced damage. Materials and Methods: Eight rams (36 ± 5 month of age and with a weight of 64 ± 7 kg) w...

the aim followed in this study was to make a comparison of the composition of fatty acids available in the sperm lipids of cattle, sheep and goat. semen samples were collected from 28 holestein cows, 12 chal rams and 7 mahabadi goats. after washing out and removal of the seminal plasma, total lipids of the sperm were extracted and fatty acids trans-methylated. the fatty acid methyl esters were ...

this study was conducted to evaluate the effect of egg yolk and cooling on ram coated spermatozoa. semen was collected from three ram by artificial vagina contacted with a tube containing tris- fructose-egg yolk 15%. samples were pooled, centrifuged by 700 g for 10 min and removed supernatant. then, samples were diluted by tris-glucose and centrifuged again to remove seminal plasma and egg yolk...

E ACH year in the central valley of California, about 10% of the breeding ewes fail to lamb. In certain years the percentage of "dry ewes" in individual flocks may be higher. Available information indicates that the problem may be environmental in nature, because a high percentage of the nonlambing ewes produce lambs in subsequent years. Because ram fertility may influence breeding efficiency u...