A technique that can be used to isolate vector/insert junctions from clones in vectors, such as yeast artificial chromosomes and P1s, and to sequence plasmid inserts more rapidly has been developed. A vector primer is combined with single, randomly chosen oligonucleotides in PCRs, to create pools of products. With 12-24 random primers used in separate reactions, a given insert junction can freq...

We have applied PCR amplification using random primers to distinguish between incipient species of the malaria vector Anopheles gambiae. Individuals belonging to three chromosomally characterized West African forms of this mosquito, which are important epidemiologically as they differ in vectorial capacity, were sampled both from laboratory stocks and from wild populations collected in three lo...

Laportea aestuans L. (Chew), Commelina virginica (L.) and Sida rhombifolia are common wild plants used in treating several ailments including diarrhea, dysentery, hernia, oedema, ulcers many more traditional African medicine especially, Nigeria. The potentials of Random Amplified polymorphic DNA (RAPD) primers delimiting intra-specific variation aestuans, C. S. was assessed using three RAPD pri...

fusarium verticillioides (f. verticillioides) is not only a primary pathogen of maize, but also can causedisease in other crops such as sorghum. pathogenicity is related to mycotoxin production such as fumonisin.in the present study, 24 isolates of f. verticillioides, which were previously identified by phenotype basedmethods, were re-identified using restriction fragment length polymorphism (r...

Anthracnose caused by Colletotrichum graminicola is one of the major diseases of sorghum. The locus for disease resistance in sorghum [Sorghum biocolor (L.) Moench] accession G73 was found to segregate as a simple recessive trait in a cross to susceptible cultivar HC136. In order to identify molecular markers linked to the locus for disease resistance, random amplified polymorphic DNA (RAPD) an...

Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP-A 1-20,...

In the present study, RAPD-PCR was used to assess genetic diversity of the rye including landrances and new rye cultivars coming from Central Europe and the Union of Soviet Socialist Republics (SUN). Five arbitrary random primers were used to determine RAPD polymorphism in the set of 38 rye genotypes. These primers amplified altogether 43 different DNA fragments with an average number of 8.6 fr...

Random amplified polymorphic DNA (RAPD) was used to study the genetic variations among strains from Fusarium sambucinum isolated from wheat in Upper Egypt. Using two different primer (V6 and M13), the used strains showed a high degree of similarities and a distinct RAPD patterns. The dendrogram that constructed based on M13 primers showed that, there is no correlation between isolation sources ...

the present study aimed to evaluate the antimicrobial activity of a hetroalkyl oximec synthetic compoundderivative (benzophenone o-4-bromoethyl oxime) against two gram positive and three gram negative bacteria.the antibacterial characteristic of this compound was shown using the circular zone diameter of bacterial growthinhibition by disk-diffusion method. in addition, the random amplified poly...

A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cult...