OBJECTIVES CmeABC is a resistance-nodulation-cell division (RND)-type multidrug efflux pump conferring resistance to clinically important antibiotics in Campylobacter. This study aimed to identify the optimal target sites for the inhibition of CmeABC with antisense peptide nucleic acid (PNA). METHODS Eighteen PNAs were designed to bind to the translational initiation regions of cmeABC, spanni...

The cohesin complex contributes to ribosome function, although the molecular mechanisms involved are unclear. Compromised cohesin function is associated with a class of diseases known as cohesinopathies. One cohesinopathy, Roberts syndrome (RBS), occurs when a mutation reduces acetylation of the cohesin Smc3 subunit. Mutation of the cohesin acetyltransferase is associated with impaired rRNA pro...

Cohesin pathway makes the necessar y arrangements C ohesin proteins arrange chromatin within the nucleus, and defects in this organizational role may underlie two human diseases, suggest Gard et al. The cohesin complex and its accessory factors are best known for holding sister chro-matids together before their separation in mitosis, but the cohesin pathway may have other functions , too. Corne...

The widespread brittle failure of welded beam-to-column connections caused by the 1994 Northridge and 1995 Kobe earthquakes highlighted the need for retrofitting measures effective in reducing the strength demand imposed on connections under cyclic loading. Researchers presented the reduced beam section (RBS) as a viable option to create a weak zone away from the connection, aiding the preventi...

Accurate estimation of terrestrial water storage anomalies (TWSA) is crucial for the sustainable management resources and human living. In this study, long-term TWSA estimates are reconstructed by integration multiple meteorological products balance (WB) method at 0.5° × resolution, generating a total 12 combinations different data. This scheme applied to 10 river basins (RBs) within China vali...

Rutherford backscattering spectroscopy (RBS) has been used during the development of multilayer thin ®lm optical ®lters, especially designed for the ̄uorescence detector of the Pierre AUGER Project. Depth pro®les of the heavy components have been measured directly, while reliable results for the light components could also be extracted indirectly. Relative thickness and density of the individua...

Time synchronization is necessary in many distributed systems, but achieving synchronization in sensornets, which combine stringent precision requirements with severe resource constraints, is particularly challenging. This challenge has been met by the recent Reference-Broadcast Synchronization (RBS) proposal, which provides ondemand pairwise synchronization with low overhead and high precision...

(Ti, Al)N/MoN and CrN/MoN multilayered films were synthesized on Si (100) surface by multi-cathodic arc ion plating system with various bilayer periods. The elemental composition and depth profiling of the films were investigated by Rutherford backscattering spectroscopy (RBS) using 2.42 and 1.52 MeV Li2+ ion beams and different incident angles (0°, 15°, 37°, and 53°). The microstructures of (T...

in this research, tellurium (te) film with thicknesses of 100-250 nm were deposited on ceramic substrates by thermal evaporation at 373 k. the thickness of the film was determined by rutherford backscattering spectroscopy. the influence of the thickness on the structural, morphological and molecular bonds was characterized using xrd, scanning electron microscope, and raman spectroscopy. the xrd...