nants containing regulatory and processing signals as powerful tools for the transfer, amplification, and subsequent analysis of foreign DNAs in mammalian cells. J. Sambrook and T. Grodzicker then present adenovirus 2-SV40 hybrids as an illustration of the potential adenovirus has as a vector for introducing exogenous sequences into eukaryotic cells. They do raise one very important considerati...

The extracellular region of the coxsackievirus and adenovirus receptor (CAR) is predicted to consist of two immunoglobulin (Ig)-related structural domains. We expressed the isolated CAR amino-terminal domain (D1) and a CAR fragment containing both extracellular Ig domains (D1/D2) in Escherichia coli. Both D1 and D1/D2 formed complexes in vitro with the recombinant knob domain of adenovirus type...

Tissue-type plasminogen activator (t-PA) is acutely released by endothelial cells. Although its endothelial storage compartment is still not well defined, t-PA release is often accompanied by release of von Willebrand factor (vWf), a protein stored in Weibel-Palade bodies. We investigated, therefore, whether t-PA is stored in these secretory organelles. Under basal culture conditions, a minorit...

Cultured COS-1 cells, as well as chicken embryonic and neonatal rat cardiac myocytes, were infected with recombinant adenovirus vectors to define limiting factors in the expression and Ca2+ transport function of recombinant sarcoplasmic-endoplasmic reticulum Ca2+ (SERCA) isoforms. Titration experiments showed that all COS-1 cells and myocytes in culture could be infected by an adenovirus titer ...

acute gastroenteritis (age) has a great impact on children less than five years of age, producing high levels of morbidity and mortality. worldwide, age is mainly related to viruses, mainly rotavirus but human adenovirus (hadv) in particular as an important etiologic agent. among hadv species f, genotypes 40 and 41 are responsible for a high number of diarrhea cases, mainly in children under 2 ...

What is adenovirus? Adenoviruses were first isolated in human adenoids, from which the name is derived. Adenoviruses are medium-sized (90–100 nm), nonenveloped (naked) icosahedral viruses composed of a nucleocapsid and linear, non-segmented double stranded (ds) DNA genome which is about 36kb long. The genes of Adenovirus are divided into two phases, early and late phases. Genes of early phase a...

In order to develop CD8+-T-cell-mediated immunotherapy against intracellular infectious agents, vaccination using recombinant virus vectors has become a promising strategy. In this study, we generated recombinant adenoviral and vaccinia virus vectors expressing a single CD8+-T-cell epitope, ANYNFTLV, which is derived from a Trypanosoma cruzi antigen. Immunogenicity of these two recombinant viru...

Tumor necrosis factor-alpha (TNF-alpha) has been intensively studied because of the specific toxicity of this cytokine toward cells that undergo malignant transformation. However, its proinflammatory and immunoregulatory properties always represented a drawback to the TNF-alpha administration in cancer therapy. In this study, we describe an adenovirus-based strategy in which the tumoricidal act...